TY - JOUR
T1 - Design aspects of bright red emissive silver nanoclusters/DNA probes for MicroRNA detection
AU - Shah, Pratik
AU - Rørvig-Lund, Andreas
AU - Chaabane, Samir Ben
AU - Thulstrup, Peter Waaben
AU - Kjaergaard, Henrik Grum
AU - Fron, Eduard
AU - Hofkens, Johan
AU - Yang, Seong Wook
AU - Vosch, Tom
PY - 2012/10/23
Y1 - 2012/10/23
N2 - The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated. The findings show that especially the propensity for mismatch self-dimer formation of the DNA probes can be a good indicator for the creation and stabilization of red emissive AgNCs. Also, the role of the thermal stability of the secondary DNA structures (mismatch self-dimer and hairpin monomers) and the observed AgNC red emission intensity were investigated. These findings can form the basis for a rationale to design new red emissive AgNC-based probes. As an example, a bright red emissive AgNC-based DNA probe was designed for RNA-miR172 detection. The latter opens the possibility to create a variety of AgNC-based DNA probes for the specific detection of plant and animal miRNAs.
AB - The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated. The findings show that especially the propensity for mismatch self-dimer formation of the DNA probes can be a good indicator for the creation and stabilization of red emissive AgNCs. Also, the role of the thermal stability of the secondary DNA structures (mismatch self-dimer and hairpin monomers) and the observed AgNC red emission intensity were investigated. These findings can form the basis for a rationale to design new red emissive AgNC-based probes. As an example, a bright red emissive AgNC-based DNA probe was designed for RNA-miR172 detection. The latter opens the possibility to create a variety of AgNC-based DNA probes for the specific detection of plant and animal miRNAs.
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U2 - 10.1021/nn302633q
DO - 10.1021/nn302633q
M3 - Article
C2 - 22947065
AN - SCOPUS:84867765190
SN - 1936-0851
VL - 6
SP - 8803
EP - 8814
JO - ACS Nano
JF - ACS Nano
IS - 10
ER -