Description of Microbacterium neungamense sp. nov. isolated from a hot spring

Dariimaa Ganbat; Ga Eul Jeong; Do Kyung Oh; Dong Woo Lee; Yong Jik Lee; Seong Bo Kim; In Tae Cha; Won Jae Chi; Seul Ki Park; Jung Sook Lee; Sang Jae Lee

doi:10.1007/s00203-022-03343-5

Description of Microbacterium neungamense sp. nov. isolated from a hot spring

Dariimaa Ganbat, Ga Eul Jeong, Do Kyung Oh, Dong Woo Lee, Yong Jik Lee, Seong Bo Kim, In Tae Cha, Won Jae Chi, Seul Ki Park, Jung Sook Lee, Sang Jae Lee

Research output: Contribution to journal › Article › peer-review

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Abstract

The Gram-positive, nonmotile, rod-shaped bacterium EF45044^T was isolated from a hot spring in Chungju, South Korea. The strain was able to grow at concentrations of 0‒5% (w/v) NaCl, at pH 6.0‒10.0 and in the temperature range of 18‒50 °C. Strain EF45044^T showed the highest 16S rRNA gene sequence similarity (98.2%) with Microbacterium ketosireducens DSM 12510^T, and the digital DNA‒DNA hybridization (dDDH), average amino acid identity (AAI), and average nucleotide identity (ANI) values were all lower than the accepted species threshold. Strain EF45044^T contained MK‒12 and MK‒13 as the predominant respiratory quinones and anteiso‒C_17:0, anteiso‒C_15:0, and iso‒C_16:0 as the major fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, and glycolipid were detected as the major polar lipids. The cell-wall peptidoglycan contained ornithine. The DNA G + C content was 71.4 mol%. Based on the polyphasic data, strain EF45044^T (= KCTC 49703^T) presents a novel species of the genus Microbacterium, for which the name Microbacterium neungamense sp. nov. is proposed.

Original language	English
Article number	23
Journal	Archives of Microbiology
Volume	205
Issue number	1
DOIs	https://doi.org/10.1007/s00203-022-03343-5
Publication status	Published - 2023 Jan

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

All Science Journal Classification (ASJC) codes

Microbiology
Biochemistry
Molecular Biology
Genetics

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title = "Description of Microbacterium neungamense sp. nov. isolated from a hot spring",

abstract = "The Gram-positive, nonmotile, rod-shaped bacterium EF45044T was isolated from a hot spring in Chungju, South Korea. The strain was able to grow at concentrations of 0‒5% (w/v) NaCl, at pH 6.0‒10.0 and in the temperature range of 18‒50 °C. Strain EF45044T showed the highest 16S rRNA gene sequence similarity (98.2%) with Microbacterium ketosireducens DSM 12510T, and the digital DNA‒DNA hybridization (dDDH), average amino acid identity (AAI), and average nucleotide identity (ANI) values were all lower than the accepted species threshold. Strain EF45044T contained MK‒12 and MK‒13 as the predominant respiratory quinones and anteiso‒C17:0, anteiso‒C15:0, and iso‒C16:0 as the major fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, and glycolipid were detected as the major polar lipids. The cell-wall peptidoglycan contained ornithine. The DNA G + C content was 71.4 mol%. Based on the polyphasic data, strain EF45044T (= KCTC 49703T) presents a novel species of the genus Microbacterium, for which the name Microbacterium neungamense sp. nov. is proposed.",

keywords = "Hot spring, Microbacterium, Microbacterium neungamense sp. nov, Polyphasic taxonomy",

author = "Dariimaa Ganbat and Jeong, {Ga Eul} and Oh, {Do Kyung} and Lee, {Dong Woo} and Lee, {Yong Jik} and Kim, {Seong Bo} and Cha, {In Tae} and Chi, {Won Jae} and Park, {Seul Ki} and Lee, {Jung Sook} and Lee, {Sang Jae}",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2023",

month = jan,

doi = "10.1007/s00203-022-03343-5",

language = "English",

volume = "205",

journal = "Archives of Microbiology",

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T1 - Description of Microbacterium neungamense sp. nov. isolated from a hot spring

AU - Ganbat, Dariimaa

AU - Jeong, Ga Eul

AU - Oh, Do Kyung

AU - Lee, Dong Woo

AU - Lee, Yong Jik

AU - Kim, Seong Bo

AU - Cha, In Tae

AU - Chi, Won Jae

AU - Park, Seul Ki

AU - Lee, Jung Sook

AU - Lee, Sang Jae

PY - 2023/1

Y1 - 2023/1

N2 - The Gram-positive, nonmotile, rod-shaped bacterium EF45044T was isolated from a hot spring in Chungju, South Korea. The strain was able to grow at concentrations of 0‒5% (w/v) NaCl, at pH 6.0‒10.0 and in the temperature range of 18‒50 °C. Strain EF45044T showed the highest 16S rRNA gene sequence similarity (98.2%) with Microbacterium ketosireducens DSM 12510T, and the digital DNA‒DNA hybridization (dDDH), average amino acid identity (AAI), and average nucleotide identity (ANI) values were all lower than the accepted species threshold. Strain EF45044T contained MK‒12 and MK‒13 as the predominant respiratory quinones and anteiso‒C17:0, anteiso‒C15:0, and iso‒C16:0 as the major fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, and glycolipid were detected as the major polar lipids. The cell-wall peptidoglycan contained ornithine. The DNA G + C content was 71.4 mol%. Based on the polyphasic data, strain EF45044T (= KCTC 49703T) presents a novel species of the genus Microbacterium, for which the name Microbacterium neungamense sp. nov. is proposed.

AB - The Gram-positive, nonmotile, rod-shaped bacterium EF45044T was isolated from a hot spring in Chungju, South Korea. The strain was able to grow at concentrations of 0‒5% (w/v) NaCl, at pH 6.0‒10.0 and in the temperature range of 18‒50 °C. Strain EF45044T showed the highest 16S rRNA gene sequence similarity (98.2%) with Microbacterium ketosireducens DSM 12510T, and the digital DNA‒DNA hybridization (dDDH), average amino acid identity (AAI), and average nucleotide identity (ANI) values were all lower than the accepted species threshold. Strain EF45044T contained MK‒12 and MK‒13 as the predominant respiratory quinones and anteiso‒C17:0, anteiso‒C15:0, and iso‒C16:0 as the major fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, and glycolipid were detected as the major polar lipids. The cell-wall peptidoglycan contained ornithine. The DNA G + C content was 71.4 mol%. Based on the polyphasic data, strain EF45044T (= KCTC 49703T) presents a novel species of the genus Microbacterium, for which the name Microbacterium neungamense sp. nov. is proposed.

KW - Hot spring

KW - Microbacterium

KW - Microbacterium neungamense sp. nov

KW - Polyphasic taxonomy

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DO - 10.1007/s00203-022-03343-5

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SN - 0302-8933

VL - 205

JO - Archives of Microbiology

JF - Archives of Microbiology

IS - 1

M1 - 23

ER -

Description of Microbacterium neungamense sp. nov. isolated from a hot spring

Abstract

Bibliographical note

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