Crystallization and preliminary X-ray crystallographic study of HP1043, a Helicobacter pylori orphan response regulator

Hyang Mi Lee; Eunmi Hong; Byoung Young Jeon; Dong Uk Kim; Jung Sue Byun; Weontae Lee; Hyun Soo Cho

doi:10.1016/j.bbapap.2005.10.024

Crystallization and preliminary X-ray crystallographic study of HP1043, a Helicobacter pylori orphan response regulator

Hyang Mi Lee, Eunmi Hong, Byoung Young Jeon, Dong Uk Kim, Jung Sue Byun, Weontae Lee, Hyun Soo Cho

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The HP1043 protein of Helicobacter pylori is related to the two-component system that regulates prokaryotic transcription in response to environmental changes. The HP1043 gene encodes an orphan response regulator (RR). The N-terminal domain of HP1043 (HP1043N) was purified by affinity-column chromatography. The purified protein was crystallized by hanging drop vapor diffusion at 293 K. The crystal belongs to primitive orthorhombic space group P2₁2₁2 with cell dimensions of a = 89.0, b = 41.3, and c = 31.7 Å. Assuming the presence of one molecule per asymmetric unit, the solvent content was estimated to be about 45%. A complete data set was collected at 1.8 Å resolution.

Original language	English
Pages (from-to)	989-991
Number of pages	3
Journal	Biochimica et Biophysica Acta - Proteins and Proteomics
Volume	1764
Issue number	5
DOIs	https://doi.org/10.1016/j.bbapap.2005.10.024
Publication status	Published - 2006 May

Bibliographical note

Funding Information:
Experiments at PLS were supported in part by MOST and POSTECH. We thank Sun-Sin Cha for assistance at beamline 6B of PLS. This work was supported in part by funds from the Korea Science and Engineering Foundation through the Protein Network Research Center and from a Korea Research Foundation Grant (KRF-2004-005-C00129), and from a Korea Research Foundation Grant funded by the Korean Government (R08-2004-000-10403-0). This work was supported by the NRL program of MOST NRDP (M1-0203-00-0020) (W.L.) from Korea Science and Engineering Foundation (KOSEF).

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biophysics
Biochemistry
Molecular Biology

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10.1016/j.bbapap.2005.10.024

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title = "Crystallization and preliminary X-ray crystallographic study of HP1043, a Helicobacter pylori orphan response regulator",

abstract = "The HP1043 protein of Helicobacter pylori is related to the two-component system that regulates prokaryotic transcription in response to environmental changes. The HP1043 gene encodes an orphan response regulator (RR). The N-terminal domain of HP1043 (HP1043N) was purified by affinity-column chromatography. The purified protein was crystallized by hanging drop vapor diffusion at 293 K. The crystal belongs to primitive orthorhombic space group P21212 with cell dimensions of a = 89.0, b = 41.3, and c = 31.7 {\AA}. Assuming the presence of one molecule per asymmetric unit, the solvent content was estimated to be about 45%. A complete data set was collected at 1.8 {\AA} resolution.",

author = "Lee, {Hyang Mi} and Eunmi Hong and Jeon, {Byoung Young} and Kim, {Dong Uk} and Byun, {Jung Sue} and Weontae Lee and Cho, {Hyun Soo}",

note = "Funding Information: Experiments at PLS were supported in part by MOST and POSTECH. We thank Sun-Sin Cha for assistance at beamline 6B of PLS. This work was supported in part by funds from the Korea Science and Engineering Foundation through the Protein Network Research Center and from a Korea Research Foundation Grant (KRF-2004-005-C00129), and from a Korea Research Foundation Grant funded by the Korean Government (R08-2004-000-10403-0). This work was supported by the NRL program of MOST NRDP (M1-0203-00-0020) (W.L.) from Korea Science and Engineering Foundation (KOSEF).",

year = "2006",

month = may,

doi = "10.1016/j.bbapap.2005.10.024",

language = "English",

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AU - Jeon, Byoung Young

AU - Kim, Dong Uk

AU - Byun, Jung Sue

AU - Lee, Weontae

AU - Cho, Hyun Soo

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Crystallization and preliminary X-ray crystallographic study of HP1043, a Helicobacter pylori orphan response regulator

Abstract

Bibliographical note

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