Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family

Byeong Gu Han; Kyung Chae Jeong; Jea Won Cho; Byung Cheon Jeong; Hyun Kyu Song; Jae Young Lee; Dong Hyuk Shin; Sangho Lee; Byung Il Lee

doi:10.1016/j.febslet.2012.04.004

Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family

Byeong Gu Han, Kyung Chae Jeong, Jea Won Cho, Byung Cheon Jeong, Hyun Kyu Song, Jae Young Lee, Dong Hyuk Shin, Sangho Lee, Byung Il Lee

Division of Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.

Original language	English
Pages (from-to)	1384-1388
Number of pages	5
Journal	FEBS Letters
Volume	586
Issue number	9
DOIs	https://doi.org/10.1016/j.febslet.2012.04.004
Publication status	Published - 2012 May 7

Bibliographical note

Funding Information:
We thank the staff at the Photon Factory (Japan) beamlines 5A and NW12A for assistance with the collection of the synchrotron data. This work was supported by the Basic Science Research Program ( 2009-0064219 and 2009-0074586 ), the Mid-career Researcher Program ( 2011-0029294 ) and the Bio & Medical Technology Development Program ( 2011-0030032 ) through the National Research Foundation of Korea (NRF), and was funded by the Ministry of Education, Science and Technology to S.L. and B.I.L.

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2012.04.004

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title = "Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family",

abstract = "Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.",

keywords = "DNA binding, DUF2666, PF2050, Pyrococcus furiosus",

author = "Han, {Byeong Gu} and Jeong, {Kyung Chae} and Cho, {Jea Won} and Jeong, {Byung Cheon} and Song, {Hyun Kyu} and Lee, {Jae Young} and Shin, {Dong Hyuk} and Sangho Lee and Lee, {Byung Il}",

note = "Funding Information: We thank the staff at the Photon Factory (Japan) beamlines 5A and NW12A for assistance with the collection of the synchrotron data. This work was supported by the Basic Science Research Program ( 2009-0064219 and 2009-0074586 ), the Mid-career Researcher Program ( 2011-0029294 ) and the Bio & Medical Technology Development Program ( 2011-0030032 ) through the National Research Foundation of Korea (NRF), and was funded by the Ministry of Education, Science and Technology to S.L. and B.I.L. ",

year = "2012",

month = may,

day = "7",

doi = "10.1016/j.febslet.2012.04.004",

language = "English",

volume = "586",

pages = "1384--1388",

journal = "FEBS Letters",

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AU - Han, Byeong Gu

AU - Jeong, Kyung Chae

AU - Cho, Jea Won

AU - Jeong, Byung Cheon

AU - Song, Hyun Kyu

AU - Lee, Jae Young

AU - Shin, Dong Hyuk

AU - Lee, Sangho

AU - Lee, Byung Il

N1 - Funding Information: We thank the staff at the Photon Factory (Japan) beamlines 5A and NW12A for assistance with the collection of the synchrotron data. This work was supported by the Basic Science Research Program ( 2009-0064219 and 2009-0074586 ), the Mid-career Researcher Program ( 2011-0029294 ) and the Bio & Medical Technology Development Program ( 2011-0030032 ) through the National Research Foundation of Korea (NRF), and was funded by the Ministry of Education, Science and Technology to S.L. and B.I.L.

PY - 2012/5/7

Y1 - 2012/5/7

N2 - Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.

AB - Pyrococcus furiosus PF2050 is an uncharacterized putative protein that contains two DUF2666 domains. Functional and structural studies of PF2050 have not previously been performed. In this study, we determined the crystal structure of PF2050. The structure of PF2050 showed that the two DUF2666 domains interact tightly, forming a globular structure. Each DUF2666 domain comprises an antiparallel β-sheet and an α-helical bundle. One side of the PF2050 structure has a positively charged basic cleft, which may have a DNA-binding function. Furthermore, we confirmed that PF2050 interacts with circular and linear dsDNA.

KW - DNA binding

KW - DUF2666

KW - PF2050

KW - Pyrococcus furiosus

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JO - FEBS Letters

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Crystal structure of Pyrococcus furiosus PF2050, a member of the DUF2666 protein family

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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