Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif

Kuglae Kim; Soon Kyeong Kwon; Sung Hoon Jun; Jeong Seok Cha; Hoyoung Kim; Weontae Lee; Jihyun F. Kim; Hyun Soo Cho

doi:10.1038/ncomms12677

Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif

Kuglae Kim, Soon Kyeong Kwon, Sung Hoon Jun, Jeong Seok Cha, Hoyoung Kim, Weontae Lee, Jihyun F. Kim, Hyun Soo Cho

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

A novel light-driven chloride-pumping rhodopsin (ClR) containing an NTQ motif' in its putative ion conduction pathway has been discovered and functionally characterized in a genomic analysis study of a marine bacterium. Here we report the crystal structure of ClR from the flavobacterium Nonlabens marinus S1-08 T determined under two conditions at 2.0 and 1.56 Å resolutions. The structures reveal two chloride-binding sites, one around the protonated Schiff base and the other on a cytoplasmic loop. We identify a 3 omega motif' formed by three non-consecutive aromatic amino acids that is correlated with the B-C loop orientation. Detailed ClR structural analyses with functional studies in E. coli reveal the chloride ion transduction pathway. Our results help understand the molecular mechanism and physiological role of ClR and provide a structural basis for optogenetic applications.

Original language	English
Article number	12677
Journal	Nature communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms12677
Publication status	Published - 2016 Aug 24

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation (NRF-2016R1A2B2013305 to H.S.C.; NRF-2011-0017670 and NRF-2014M3C9A3068822 to J.F.K.; and NRF-2013R1A2A2A01068963 to W.L.) of the Ministry of Science, ICT and Future Planning, and the Strategic Initiative for Microbiomes in Agriculture of the Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. The work was supported in part by Brain Korea 21 (BK21) PLUS program/S.-K.K., S.-H.J., J.S.C. and H.K. are fellowship awardees of BK21 PLUS program.

Publisher Copyright:
© The Author(s) 2016.

All Science Journal Classification (ASJC) codes

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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title = "Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif",

abstract = "A novel light-driven chloride-pumping rhodopsin (ClR) containing an NTQ motif' in its putative ion conduction pathway has been discovered and functionally characterized in a genomic analysis study of a marine bacterium. Here we report the crystal structure of ClR from the flavobacterium Nonlabens marinus S1-08 T determined under two conditions at 2.0 and 1.56 {\AA} resolutions. The structures reveal two chloride-binding sites, one around the protonated Schiff base and the other on a cytoplasmic loop. We identify a 3 omega motif' formed by three non-consecutive aromatic amino acids that is correlated with the B-C loop orientation. Detailed ClR structural analyses with functional studies in E. coli reveal the chloride ion transduction pathway. Our results help understand the molecular mechanism and physiological role of ClR and provide a structural basis for optogenetic applications.",

author = "Kuglae Kim and Kwon, {Soon Kyeong} and Jun, {Sung Hoon} and Cha, {Jeong Seok} and Hoyoung Kim and Weontae Lee and Kim, {Jihyun F.} and Cho, {Hyun Soo}",

note = "Funding Information: This work was supported by the National Research Foundation (NRF-2016R1A2B2013305 to H.S.C.; NRF-2011-0017670 and NRF-2014M3C9A3068822 to J.F.K.; and NRF-2013R1A2A2A01068963 to W.L.) of the Ministry of Science, ICT and Future Planning, and the Strategic Initiative for Microbiomes in Agriculture of the Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. The work was supported in part by Brain Korea 21 (BK21) PLUS program/S.-K.K., S.-H.J., J.S.C. and H.K. are fellowship awardees of BK21 PLUS program. Publisher Copyright: {\textcopyright} The Author(s) 2016.",

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AU - Cho, Hyun Soo

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N2 - A novel light-driven chloride-pumping rhodopsin (ClR) containing an NTQ motif' in its putative ion conduction pathway has been discovered and functionally characterized in a genomic analysis study of a marine bacterium. Here we report the crystal structure of ClR from the flavobacterium Nonlabens marinus S1-08 T determined under two conditions at 2.0 and 1.56 Å resolutions. The structures reveal two chloride-binding sites, one around the protonated Schiff base and the other on a cytoplasmic loop. We identify a 3 omega motif' formed by three non-consecutive aromatic amino acids that is correlated with the B-C loop orientation. Detailed ClR structural analyses with functional studies in E. coli reveal the chloride ion transduction pathway. Our results help understand the molecular mechanism and physiological role of ClR and provide a structural basis for optogenetic applications.

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Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif

Abstract

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