TY - JOUR
T1 - Crossed immunoelectrophoretic analysis of Legionella pneumophila serogroup 1 antigens
AU - Collins, Michael T.
AU - Cho, Sang Nae
AU - Høiby, Niels
AU - Espersen, Frank
AU - Baek, Leif
AU - Reif, J. S.
PY - 1983
Y1 - 1983
N2 - By crossed immunoelectrophoresis, 85 different antigens were demonstrated in sonicated preparations of Legionella pneumophila serogroup 1 (Lp1). The precipitin patterns of 82 anodic-migrating antigens were numbered and were designated the Lp1 reference system. Eleven antigens were stable to boiling, and 7 of these were shown to be surface antigens. One heat-stable surface antigen (antigen no. 61) was highly reactive with limulus amoebocyte lysates and formed a precipitin resembling lipopolysaccharide. Serum from an isolation confirmed case of Lp1 infection and serogroup-specific rabbit antiserum reacted specifically with antigen no. 61, which was designated the serogroup-specific antigen. Normal human and rabbit sera commonly had antibodies to antigen no. 66 of the Lp1 reference system. This antigen is antigenically related to the 'common antigen' of Pseudomonas aeruginosa.
AB - By crossed immunoelectrophoresis, 85 different antigens were demonstrated in sonicated preparations of Legionella pneumophila serogroup 1 (Lp1). The precipitin patterns of 82 anodic-migrating antigens were numbered and were designated the Lp1 reference system. Eleven antigens were stable to boiling, and 7 of these were shown to be surface antigens. One heat-stable surface antigen (antigen no. 61) was highly reactive with limulus amoebocyte lysates and formed a precipitin resembling lipopolysaccharide. Serum from an isolation confirmed case of Lp1 infection and serogroup-specific rabbit antiserum reacted specifically with antigen no. 61, which was designated the serogroup-specific antigen. Normal human and rabbit sera commonly had antibodies to antigen no. 66 of the Lp1 reference system. This antigen is antigenically related to the 'common antigen' of Pseudomonas aeruginosa.
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U2 - 10.1128/iai.39.3.1428-1440.1983
DO - 10.1128/iai.39.3.1428-1440.1983
M3 - Article
C2 - 6840845
AN - SCOPUS:0020683791
SN - 1094-2025
VL - 39
SP - 1428
EP - 1440
JO - Review of Economic Dynamics
JF - Review of Economic Dynamics
IS - 3
ER -
