Continuous biohydrogen production in a CSTR using starch as a substrate

Muhammad Farhan Arooj, Sun Kee Han, Sang Hyoun Kim, Dong Hoon Kim, Hang Sik Shin

Research output: Contribution to journalArticlepeer-review

141 Citations (Scopus)


There are few reports on continuous biohydrogen production from biopolymers like starch, because most previous studies on continuous operation were performed using simple hydrolysates such as glucose and sucrose. This study was, therefore, carried out to investigate continuous biohydrogen production in an anaerobic continuous stirred-tank reactor (CSTR) using corn starch as a substrate for 158 days. The reactor was seeded with mixed community sludge from an anaerobic digester at a local wastewater treatment facility. Volatile fatty acids (VFA) profile supported the fact that butyrate to acetate (B/A) ratio was the most important parameter to justify hydrogen yield at various hydraulic retention times (HRT). Maximum hydrogen yield of 0.92 mol H2/mol glucoseadded was observed at 12 h HRT with the highest B/A ratio of 4.3. Also, estimation modeling to quantify homoacetogenism was conducted and H2-consuming acetate production at various HRTs was estimated. Estimation modeling revealed that at short HRTs of 9, 6 and 4 h, acetate concentration plays a trivial role. Therefore, butyrate concentration alone instead of butyrate to acetate ratio (B/A) justifies the trend of H2 yield at short HRTs.

Original languageEnglish
Pages (from-to)3289-3294
Number of pages6
JournalInternational Journal of Hydrogen Energy
Issue number13
Publication statusPublished - 2008 Jul

Bibliographical note

Funding Information:
This work was supported by Grant no. M1-0203-00-0063 by National Research Laboratory Program of Korean Ministry of Science and Technology.

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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