Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies

Ji Hong Bong; Tae Hun Kim; Jaeyong Jung; Soo Jeong Lee; Jeong Soo Sung; Chang Kyu Lee; Min Jung Kang; Hyun Ok Kim; Jae Chul Pyun

doi:10.1007/s13206-021-00011-6

Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies

Ji Hong Bong, Tae Hun Kim, Jaeyong Jung, Soo Jeong Lee, Jeong Soo Sung, Chang Kyu Lee, Min Jung Kang, Hyun Ok Kim, Jae Chul Pyun

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.

Original language	English
Pages (from-to)	100-108
Number of pages	9
Journal	Biochip Journal
Volume	15
Issue number	1
DOIs	https://doi.org/10.1007/s13206-021-00011-6
Publication status	Published - 2021 Mar

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea [grant number: NRF-2020R1A2B5B01002187 and NRF-2020R1A5A101913111.

Publisher Copyright:
© 2021, The Korean BioChip Society.

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Electrical and Electronic Engineering

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10.1007/s13206-021-00011-6

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title = "Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies",

abstract = "Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.",

author = "Bong, {Ji Hong} and Kim, {Tae Hun} and Jaeyong Jung and Lee, {Soo Jeong} and Sung, {Jeong Soo} and Lee, {Chang Kyu} and Kang, {Min Jung} and Kim, {Hyun Ok} and Pyun, {Jae Chul}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea [grant number: NRF-2020R1A2B5B01002187 and NRF-2020R1A5A101913111. Publisher Copyright: {\textcopyright} 2021, The Korean BioChip Society.",

year = "2021",

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doi = "10.1007/s13206-021-00011-6",

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AU - Bong, Ji Hong

AU - Kim, Tae Hun

AU - Jung, Jaeyong

AU - Lee, Soo Jeong

AU - Sung, Jeong Soo

AU - Lee, Chang Kyu

AU - Kang, Min Jung

AU - Kim, Hyun Ok

AU - Pyun, Jae Chul

N1 - Funding Information: This work was supported by the National Research Foundation of Korea [grant number: NRF-2020R1A2B5B01002187 and NRF-2020R1A5A101913111. Publisher Copyright: © 2021, The Korean BioChip Society.

PY - 2021/3

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N2 - Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.

AB - Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.

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Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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