Comparison of the immunoCAP assay and advansure™ alloscreen advanced multiplex specific IgE detection assay

Kyung Hee Park; Jongsun Lee; Sang Chul Lee; Young Woong Son; Da Woon Sim; Jae Hyun Lee; Jung Won Park

doi:10.3349/ymj.2017.58.4.786

Comparison of the immunoCAP assay and advansure™ alloscreen advanced multiplex specific IgE detection assay

Kyung Hee Park, Jongsun Lee, Sang Chul Lee, Young Woong Son, Da Woon Sim, Jae Hyun Lee, Jung Won Park

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Purpose: The AdvanSure™ AlloScreen assay is an advanced multiplex test that allows for simultaneous detection of specific IgE (sIgE) against multiple allergens. For precise identification of causative allergens in allergic patients, we compared this new multiplex sIgE assay with the ImmunoCAP assay, which is currently the gold-standard method for sIgE detection. Materials and Methods: Serum samples from 218 Korean allergic disease patients were used to compare the ImmunoCAP and AlloScreen assays with respect to the following 13 allergens: Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat and dog dander, Alternaria, birch, oak, ragweed, mugwort, rye grass, and food allergens (egg white, cow’s milk, peanuts). Results: A total of 957 paired tests using the 13 allergens were compared. The total agreement ratio ranged from 0.74 (oak) to 0.97 (Alternaria). With respect to class association analyses, the gamma index ranged from 0.819 (rye grass) to 0.990 (Alternaria). The intra-class correlation coefficients for house dust mites, cat and dog dander, Alternaria, birch, ragweed, egg white, cow’s milk, and peanut sIgE titers were >0.8. Conclusion: The AlloScreen and ImmunoCAP assays exhibited similar diagnostic performance. However, due to methodological differences between the two systems, careful interpretation of their results is needed in clinical applications.

Original language	English
Pages (from-to)	786-792
Number of pages	7
Journal	Yonsei medical journal
Volume	58
Issue number	4
DOIs	https://doi.org/10.3349/ymj.2017.58.4.786
Publication status	Published - 2017 Jul

Bibliographical note

Funding Information:
This research was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute, funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI13C0010) and LG Life Science (1-2014-0068). The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Publisher Copyright:
© Yonsei University College of Medicine 2017.

All Science Journal Classification (ASJC) codes

Medicine(all)

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10.3349/ymj.2017.58.4.786

Cite this

@article{e949feed37df42899acf2e3bf31bd85e,

title = "Comparison of the immunoCAP assay and advansure{\texttrademark} alloscreen advanced multiplex specific IgE detection assay",

abstract = "Purpose: The AdvanSure{\texttrademark} AlloScreen assay is an advanced multiplex test that allows for simultaneous detection of specific IgE (sIgE) against multiple allergens. For precise identification of causative allergens in allergic patients, we compared this new multiplex sIgE assay with the ImmunoCAP assay, which is currently the gold-standard method for sIgE detection. Materials and Methods: Serum samples from 218 Korean allergic disease patients were used to compare the ImmunoCAP and AlloScreen assays with respect to the following 13 allergens: Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat and dog dander, Alternaria, birch, oak, ragweed, mugwort, rye grass, and food allergens (egg white, cow{\textquoteright}s milk, peanuts). Results: A total of 957 paired tests using the 13 allergens were compared. The total agreement ratio ranged from 0.74 (oak) to 0.97 (Alternaria). With respect to class association analyses, the gamma index ranged from 0.819 (rye grass) to 0.990 (Alternaria). The intra-class correlation coefficients for house dust mites, cat and dog dander, Alternaria, birch, ragweed, egg white, cow{\textquoteright}s milk, and peanut sIgE titers were >0.8. Conclusion: The AlloScreen and ImmunoCAP assays exhibited similar diagnostic performance. However, due to methodological differences between the two systems, careful interpretation of their results is needed in clinical applications.",

author = "Park, {Kyung Hee} and Jongsun Lee and Lee, {Sang Chul} and Son, {Young Woong} and Sim, {Da Woon} and Lee, {Jae Hyun} and Park, {Jung Won}",

note = "Funding Information: This research was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute, funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI13C0010) and LG Life Science (1-2014-0068). The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication. Publisher Copyright: {\textcopyright} Yonsei University College of Medicine 2017.",

year = "2017",

month = jul,

doi = "10.3349/ymj.2017.58.4.786",

language = "English",

volume = "58",

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AU - Park, Kyung Hee

AU - Lee, Jongsun

AU - Lee, Sang Chul

AU - Son, Young Woong

AU - Sim, Da Woon

AU - Lee, Jae Hyun

AU - Park, Jung Won

N1 - Funding Information: This research was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute, funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI13C0010) and LG Life Science (1-2014-0068). The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication. Publisher Copyright: © Yonsei University College of Medicine 2017.

PY - 2017/7

Y1 - 2017/7

N2 - Purpose: The AdvanSure™ AlloScreen assay is an advanced multiplex test that allows for simultaneous detection of specific IgE (sIgE) against multiple allergens. For precise identification of causative allergens in allergic patients, we compared this new multiplex sIgE assay with the ImmunoCAP assay, which is currently the gold-standard method for sIgE detection. Materials and Methods: Serum samples from 218 Korean allergic disease patients were used to compare the ImmunoCAP and AlloScreen assays with respect to the following 13 allergens: Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat and dog dander, Alternaria, birch, oak, ragweed, mugwort, rye grass, and food allergens (egg white, cow’s milk, peanuts). Results: A total of 957 paired tests using the 13 allergens were compared. The total agreement ratio ranged from 0.74 (oak) to 0.97 (Alternaria). With respect to class association analyses, the gamma index ranged from 0.819 (rye grass) to 0.990 (Alternaria). The intra-class correlation coefficients for house dust mites, cat and dog dander, Alternaria, birch, ragweed, egg white, cow’s milk, and peanut sIgE titers were >0.8. Conclusion: The AlloScreen and ImmunoCAP assays exhibited similar diagnostic performance. However, due to methodological differences between the two systems, careful interpretation of their results is needed in clinical applications.

AB - Purpose: The AdvanSure™ AlloScreen assay is an advanced multiplex test that allows for simultaneous detection of specific IgE (sIgE) against multiple allergens. For precise identification of causative allergens in allergic patients, we compared this new multiplex sIgE assay with the ImmunoCAP assay, which is currently the gold-standard method for sIgE detection. Materials and Methods: Serum samples from 218 Korean allergic disease patients were used to compare the ImmunoCAP and AlloScreen assays with respect to the following 13 allergens: Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat and dog dander, Alternaria, birch, oak, ragweed, mugwort, rye grass, and food allergens (egg white, cow’s milk, peanuts). Results: A total of 957 paired tests using the 13 allergens were compared. The total agreement ratio ranged from 0.74 (oak) to 0.97 (Alternaria). With respect to class association analyses, the gamma index ranged from 0.819 (rye grass) to 0.990 (Alternaria). The intra-class correlation coefficients for house dust mites, cat and dog dander, Alternaria, birch, ragweed, egg white, cow’s milk, and peanut sIgE titers were >0.8. Conclusion: The AlloScreen and ImmunoCAP assays exhibited similar diagnostic performance. However, due to methodological differences between the two systems, careful interpretation of their results is needed in clinical applications.

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Comparison of the immunoCAP assay and advansure™ alloscreen advanced multiplex specific IgE detection assay

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