TY - JOUR
T1 - Comparison of fatty acid analysis methods for assessing biorefinery applicability of wastewater cultivated microalgae
AU - Woo, Sung Geun
AU - Yoo, Keunje
AU - Lee, Juyoun
AU - Bang, Sara
AU - Lee, Myungjin
AU - On, Kwangchul
AU - Park, Joonhong
N1 - Funding Information:
This work was supported by the Mid-career Researcher Program through an NRF grant funded by the MEST (No. 2009-0081153 ).
PY - 2012/8/15
Y1 - 2012/8/15
N2 - In this study, we compared the performances of four different gas chromatography (GC) based microalgal fatty acid analysis methods that are typically applied to biorefinery research using wastewater-adapted microalgae. Compared with the HP-5-type non-polar column, WAX-type polar columns exhibited excellent abilities to quantitatively separate C16-C18 polyunsaturated fatty acids (PUFAs) from selected wastewater-adapted microalgae (Chlorella vulgaris, Ankistrodesmus gracilis and Scenedesmus quadricauda) isolates. GC-mass spectroscopy (MS) using the WAX-type polar column provided the strongest detection sensitivity among the tested methods by lowest detection limit, and GC-flame ionized detector (FID) with the same polar column exhibited nearly consistent results to GC-MS analysis. Our statistical comparison of microalgal fatty acid composition profiles generated using various GC methods, microalgal resources and culture media (wastewater, BG11 and nitrogen limitation) suggested that an appropriate GC method and algal resource choice are more important than the optimization of culture conditions to evaluate the applicability of microalgal biorefinery using wastewater resources.
AB - In this study, we compared the performances of four different gas chromatography (GC) based microalgal fatty acid analysis methods that are typically applied to biorefinery research using wastewater-adapted microalgae. Compared with the HP-5-type non-polar column, WAX-type polar columns exhibited excellent abilities to quantitatively separate C16-C18 polyunsaturated fatty acids (PUFAs) from selected wastewater-adapted microalgae (Chlorella vulgaris, Ankistrodesmus gracilis and Scenedesmus quadricauda) isolates. GC-mass spectroscopy (MS) using the WAX-type polar column provided the strongest detection sensitivity among the tested methods by lowest detection limit, and GC-flame ionized detector (FID) with the same polar column exhibited nearly consistent results to GC-MS analysis. Our statistical comparison of microalgal fatty acid composition profiles generated using various GC methods, microalgal resources and culture media (wastewater, BG11 and nitrogen limitation) suggested that an appropriate GC method and algal resource choice are more important than the optimization of culture conditions to evaluate the applicability of microalgal biorefinery using wastewater resources.
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U2 - 10.1016/j.talanta.2012.04.002
DO - 10.1016/j.talanta.2012.04.002
M3 - Article
C2 - 22841053
AN - SCOPUS:84864361632
SN - 0039-9140
VL - 97
SP - 103
EP - 110
JO - Talanta
JF - Talanta
ER -