Comparison between newly developed and commercial inhalant skin prick test reagents using in vivo and in vitro methods

Sang Chul Lee, Da Woon Sim, Jongsun Lee, Kyoung Yong Jeong, Kyung Hee Park, Jae Hyun Lee, Jung Dong Kim, Jung Won Park

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8 Citations (Scopus)


Background: We developed skin prick test (SPT) reagents for common inhalant allergens that reflected the real exposure in Korea. The study aim was to evaluate diagnostic usefulness and allergen potency of our inhalant SPT reagents in comparison with commercial products. Methods: We produced eight common inhalant allergen SPT reagents using total extract (Prolagen): Dermatophagoides farinae, Dermatophagoides pteronyssinus, oak, ragweed, mugwort, Humulus japonicus pollens, as well as cat and dog allergens. We compared the newly developed reagents with three commercially available SPT reagents (Allergopharma, Hollister-Stier, Lofarma). We measured total protein concentrations, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), major allergen concentration, and biological allergen potencies measured by immunoglobulin E (IgE) immunoblotting and ImmunoCAP inhibition test. Results: Diagnostic values of these SPT reagents were expressed as positivity rate and concordance rate of the results from ImmunoCAP allergen-specific IgE test in 94 allergic patients. In vitro analysis showed marked differences in protein concentrations, SDS-PAGE features, major allergen concentrations, and biological allergen potencies of four different SPT reagents. In vivo analysis showed that positive rates and concordance rates of Prolagen® SPT reagents were similar compared to the three commercial SPT reagents. Conclusion: The newly developed Prolagen® inhalant SPT reagents are not inferior to the commercially available SPT reagents in allergy diagnosis.

Original languageEnglish
Article numbere101
JournalJournal of Korean medical science
Issue number13
Publication statusPublished - 2018 Mar 26

Bibliographical note

Publisher Copyright:
© 2018 The Korean Academy of Medical Sciences.

All Science Journal Classification (ASJC) codes

  • Medicine(all)


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