Comparative study on resolution enhancements in fluorescence-structured illumination Fresnel incoherent correlation holography

Philjun Jeon; Jongwu Kim; Heejung Lee; Hyuk Sang Kwon; Dug Young Kim

doi:10.1364/OE.417206

Comparative study on resolution enhancements in fluorescence-structured illumination Fresnel incoherent correlation holography

Philjun Jeon, Jongwu Kim, Heejung Lee, Hyuk Sang Kwon, Dug Young Kim

Department of Physics

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Fresnel incoherent correlation holography (FINCH) is a new approach for incoherent holography, which also has enhancement in the transverse resolution. Structured illumination microscopy (SIM) is another promising super-resolution technique. SI-FINCH, the combination of SIM and FINCH, has been demonstrated lately for scattering objects. In this study, we extended the application of SI-FINCH toward fluorescent microscopy. We have built a versatile multimodal microscopy system that can obtain images of four different imaging schemes: conventional fluorescence microscopy, FINCH, SIM, and SI-FINCH. Resolution enhancements were demonstrated by comparing the point spread functions (PSFs) of the four different imaging systems by using fluorescence beads of 1-µm diameter.

Original language	English
Pages (from-to)	9231-9241
Number of pages	11
Journal	Optics Express
Volume	29
Issue number	6
DOIs	https://doi.org/10.1364/OE.417206
Publication status	Published - 2021 Mar 15

Bibliographical note

Funding Information:
National Research Foundation of Korea (2017R1A2B4003950); Korea Institute for Advancement of Technology (P0011925).

Publisher Copyright:
© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

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10.1364/OE.417206

Cite this

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title = "Comparative study on resolution enhancements in fluorescence-structured illumination Fresnel incoherent correlation holography",

abstract = "Fresnel incoherent correlation holography (FINCH) is a new approach for incoherent holography, which also has enhancement in the transverse resolution. Structured illumination microscopy (SIM) is another promising super-resolution technique. SI-FINCH, the combination of SIM and FINCH, has been demonstrated lately for scattering objects. In this study, we extended the application of SI-FINCH toward fluorescent microscopy. We have built a versatile multimodal microscopy system that can obtain images of four different imaging schemes: conventional fluorescence microscopy, FINCH, SIM, and SI-FINCH. Resolution enhancements were demonstrated by comparing the point spread functions (PSFs) of the four different imaging systems by using fluorescence beads of 1-µm diameter.",

author = "Philjun Jeon and Jongwu Kim and Heejung Lee and Kwon, {Hyuk Sang} and Kim, {Dug Young}",

note = "Funding Information: National Research Foundation of Korea (2017R1A2B4003950); Korea Institute for Advancement of Technology (P0011925). Publisher Copyright: {\textcopyright} 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement",

year = "2021",

month = mar,

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doi = "10.1364/OE.417206",

language = "English",

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journal = "Optics Express",

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AU - Jeon, Philjun

AU - Kim, Jongwu

AU - Lee, Heejung

AU - Kwon, Hyuk Sang

AU - Kim, Dug Young

N1 - Funding Information: National Research Foundation of Korea (2017R1A2B4003950); Korea Institute for Advancement of Technology (P0011925). Publisher Copyright: © 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

PY - 2021/3/15

Y1 - 2021/3/15

N2 - Fresnel incoherent correlation holography (FINCH) is a new approach for incoherent holography, which also has enhancement in the transverse resolution. Structured illumination microscopy (SIM) is another promising super-resolution technique. SI-FINCH, the combination of SIM and FINCH, has been demonstrated lately for scattering objects. In this study, we extended the application of SI-FINCH toward fluorescent microscopy. We have built a versatile multimodal microscopy system that can obtain images of four different imaging schemes: conventional fluorescence microscopy, FINCH, SIM, and SI-FINCH. Resolution enhancements were demonstrated by comparing the point spread functions (PSFs) of the four different imaging systems by using fluorescence beads of 1-µm diameter.

AB - Fresnel incoherent correlation holography (FINCH) is a new approach for incoherent holography, which also has enhancement in the transverse resolution. Structured illumination microscopy (SIM) is another promising super-resolution technique. SI-FINCH, the combination of SIM and FINCH, has been demonstrated lately for scattering objects. In this study, we extended the application of SI-FINCH toward fluorescent microscopy. We have built a versatile multimodal microscopy system that can obtain images of four different imaging schemes: conventional fluorescence microscopy, FINCH, SIM, and SI-FINCH. Resolution enhancements were demonstrated by comparing the point spread functions (PSFs) of the four different imaging systems by using fluorescence beads of 1-µm diameter.

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Comparative study on resolution enhancements in fluorescence-structured illumination Fresnel incoherent correlation holography

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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