Comparative evaluation of three homogenization methods for isolating middle east respiratory syndrome coronavirus nucleic acids from sputum samples for real-time reverse transcription PCR

Medicine and Dentistry

• Sputum 100%
• Phosphate Buffered Saline 50%
• Middle East Respiratory Syndrome Coronavirus 50%
• RNA 40%
• Acetic Acid Derivative 40%
• Cysteine 40%
• Citrate Sodium 40%
• Nucleic Acid 30%
• Deoxyribonuclease 30%
• Proteinase K 30%
• Therapeutic Procedure 30%
• Diagnosis 20%
• Quantitative Reverse Transcription Polymerase Chain Reaction 20%
• Culture 20%
• Reverse Transcription Polymerase Chain Reaction 10%
• Mucus 10%
• False Negative Result 10%
• Evaluation Study 10%
• Coronavirus Infection 10%