Comparative evaluation of hydrogen fermentation of de-oiled Jatropha waste hydrolyzates

Fermentation of de-oiled Jatropha waste (DJW) hydrolyzate was carried out at 55 °C and pH 7 to evaluate the efficacy of the DJW pretreatment methods for enhancing the hydrogen production rate. The enzyme (7.5%) treated hydrolyzate showed a peak hydrogen production rate (HPR) and hydrogen yield (HY) of 5146 mL H₂/L-d and 98 mL H₂/g VS_added, respectively. The HCl (2.5%), H₂SO₄ (0.5%), ultrasonication (45 min), and heat treatment (100 °C, 45 min) treatments gave maximal HY of 43, 24, 23, and 34 mL H₂/g VS_added, respectively. Further enzyme (5%) treatment of the acid (2%) unhydrolyzed biomass showed HPR of 2947 mL H₂/L-d and HY of 106 mL H₂/g VS_added. Besides, it was also observed that higher DJW concentrations during pretreatment, and higher butyrate levels in the fermentation process, led to increased release of reducing sugar and increased HPR, respectively. The total bioenergy production analysis revealed nearly 10-fold increase in the energy values with peak HPR and HY of 55 kJ/L-d and 1.0 kJ/g VS_added, respectively.