The current study comparatively evaluated the effects of silver nanoparticles (AgNPs) on fermentative bio hydrogen (bio-H2) production by Clostridium beijerinckii (KTCC1737). Green synthesized AgNPs were fabricated using henna (Lawsonia inermis) and DU-C2 actinomycete in batch using different AgNPs concentrations (0, 40, 80, and 120 μg/mL). The overall results revealed that after 72 hours of AgNPs incubation with C beijerinckii, the henna mediated AgNP synthesis increased the hydrogen production rate with a hydrogen yield of 1.71 mol H2/mol glucose added. However, the DU-C2-mediated AgNps synthesis inhibited bio-H2 production during the batch H2 fermentation. Performed live/dead staining showed AgNPs effect on the bacterial culture in batch fermentation. The results indicated that the henna mediated AgNPs enhanced the bacterial cell population, meanwhile, in case of DU-C2 AgNPs, less bacterial population occurred. Thus, the present study provides a clear overview on the application of metallic NPs as H2 production catalysts to further improve bio-H2 production.
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© 2020 John Wiley & Sons Ltd
All Science Journal Classification (ASJC) codes
- Renewable Energy, Sustainability and the Environment
- Nuclear Energy and Engineering
- Fuel Technology
- Energy Engineering and Power Technology