Comparative analysis of ABCG2-expressing and label-retaining cells in mouse submandibular gland

Yeun Jung Kim, Hyuk Jae Kwon, Naoshi Shinozaki, Sadamitsu Hashimoto, Masaki Shimono, Sung Won Cho, Han Sung Jung

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13 Citations (Scopus)


The submandibular gland (SMG) is a tissue that can be regenerated in a tissue injury model and that has adult stem cells capable of self-renewal and differentiation into functional cells. We have analyzed the localization of label-retaining cells (LRCs), which are putative progenitor cells, by using the BrdU-labeling method. 5-Bromo-2′-deoxyuridine (BrdU) injection followed by a long chasing period permitted the identification of LRCs based on the slow-cycling characteristic. In order to confirm the accurate localization of LRCs, BrdU and SMG-specific markers, including aquaporin5, cytokeratin, and smooth muscle actin, were examined by double-immunofluoresence staining. We found that LRCs were distributed in the acinus, duct, myoepithelium, and connective tissue. Moreover, ABCG2 (a known stem cell marker) was used for the characterization of LRCs and the localization of cells as putative stem/progenitor cells. ABCG2-expressing cells were distributed in various regions of the SMG but did not co-localize with LRCs. We suggest that putative progenitor cells exist in various regions of the SMG and have diverse capacities to differentiate into specific cells.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalCell and Tissue Research
Issue number1
Publication statusPublished - 2008 Oct

Bibliographical note

Funding Information:
This work was supported by Korea Research Foundation Grant (KRF-2006–013-E00143). Y.-J.Kim.H.-J.Kwon.S.-W.Cho.H.-S.Jung(*) Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, 134 Shinchon-Dong, Seodaemoon-Gu, Seoul 120-752, Korea e-mail:

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology


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