Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging

Donghak Lee, Suho Ryu, Uihan Kim, Daeseong Jung, Chulmin Joo

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)


We present a multi-contrast microscope based on color-coded illumination and computation. A programmable three-color light-emitting diode (LED) array illuminates a specimen, in which each color corresponds to a different illumination angle. A single color image sensor records light transmitted through the specimen, and images at each color channel are then separated and utilized to obtain bright-field, dark-field, and differential phase contrast (DPC) images simultaneously. Quantitative phase imaging is also achieved based on DPC images acquired with two different LED illumination patterns. The multi-contrast and quantitative phase imaging capabilities of our method are demonstrated by presenting images of various transparent biological samples.

Original languageEnglish
Article number249958
Pages (from-to)4912-4922
Number of pages11
JournalBiomedical Optics Express
Issue number12
Publication statusPublished - 2015 Nov 18

Bibliographical note

Publisher Copyright:
© 2015 Optical Society of America.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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