Colloidal gold nanoparticle conjugates of gefitinib

Anh Thu Ngoc Lam; Jinha Yoon; Erdene Ochir Ganbold; Dheeraj K. Singh; Doseok Kim; Kwang Hwi Cho; So Yeong Lee; Jaebum Choo; Kangtaek Lee; Sang Woo Joo

doi:10.1016/j.colsurfb.2014.08.021

Colloidal gold nanoparticle conjugates of gefitinib

Anh Thu Ngoc Lam, Jinha Yoon, Erdene Ochir Ganbold, Dheeraj K. Singh, Doseok Kim, Kwang Hwi Cho, So Yeong Lee, Jaebum Choo, Kangtaek Lee, Sang Woo Joo

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Gefitinib (GF) is a US Food and Drug Administration-approved epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor for treating the lung cancers. We fabricated colloidal gold nanoparticle (AuNP) conjugates of the GF anticancer drug by self-assembly to test their potency against A549, NCI-H460, and NCI-H1975 lung cancer cells. GF adsorption on AuNP surfaces was examined by UV-vis absorption spectra and surface-enhanced Raman scattering. Density functional theory calculations were performed to estimate the energetic stabilities of the drug-AuNP composites. The N1 nitrogen atom of the quinazoline ring of GF was calculated to be more stable than the N3 in binding Au cluster atoms. The internalizations of GF-coated AuNPs were examined by transmission electron and dark-field microscopy. A cell viability test of AuNP-GF conjugates with the EGFR antibody exhibited much higher reductions than free GF for A549, NCI-H460, and NCI-H1975 lung cancer cells after treatment for 48. h.

Original language	English
Pages (from-to)	61-67
Number of pages	7
Journal	Colloids and Surfaces B: Biointerfaces
Volume	123
DOIs	https://doi.org/10.1016/j.colsurfb.2014.08.021
Publication status	Published - 2014 Nov 1

Bibliographical note

Funding Information:
We would like to thank Semi Kim for her help on TEM measurements. SWJ, Prof. So Yeong Lee, and KL thank Dr. Kyong-Ah Yoon for their helpful comments on the EGFR mutations. Prof. K. Cho acknowledges the support by the Human Resources Development of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government Ministry of Trade, Industry & Energy (No. 20124030200070 ). Prof. D. Kim acknowledges support from the Korea government (MEST) No. 2011-0031496 . Prof. J. Choo acknowledges that the Nano Material Technology Development Program supported this work through the National Research Foundation of Korea, funded by the Ministry of Education, Science, and Technology (grant number 2012035286 ).

Publisher Copyright:
© 2014 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.colsurfb.2014.08.021

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title = "Colloidal gold nanoparticle conjugates of gefitinib",

abstract = "Gefitinib (GF) is a US Food and Drug Administration-approved epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor for treating the lung cancers. We fabricated colloidal gold nanoparticle (AuNP) conjugates of the GF anticancer drug by self-assembly to test their potency against A549, NCI-H460, and NCI-H1975 lung cancer cells. GF adsorption on AuNP surfaces was examined by UV-vis absorption spectra and surface-enhanced Raman scattering. Density functional theory calculations were performed to estimate the energetic stabilities of the drug-AuNP composites. The N1 nitrogen atom of the quinazoline ring of GF was calculated to be more stable than the N3 in binding Au cluster atoms. The internalizations of GF-coated AuNPs were examined by transmission electron and dark-field microscopy. A cell viability test of AuNP-GF conjugates with the EGFR antibody exhibited much higher reductions than free GF for A549, NCI-H460, and NCI-H1975 lung cancer cells after treatment for 48. h.",

author = "Lam, {Anh Thu Ngoc} and Jinha Yoon and Ganbold, {Erdene Ochir} and Singh, {Dheeraj K.} and Doseok Kim and Cho, {Kwang Hwi} and Lee, {So Yeong} and Jaebum Choo and Kangtaek Lee and Joo, {Sang Woo}",

note = "Funding Information: We would like to thank Semi Kim for her help on TEM measurements. SWJ, Prof. So Yeong Lee, and KL thank Dr. Kyong-Ah Yoon for their helpful comments on the EGFR mutations. Prof. K. Cho acknowledges the support by the Human Resources Development of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government Ministry of Trade, Industry & Energy (No. 20124030200070 ). Prof. D. Kim acknowledges support from the Korea government (MEST) No. 2011-0031496 . Prof. J. Choo acknowledges that the Nano Material Technology Development Program supported this work through the National Research Foundation of Korea, funded by the Ministry of Education, Science, and Technology (grant number 2012035286 ). Publisher Copyright: {\textcopyright} 2014 Elsevier B.V.",

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AU - Lam, Anh Thu Ngoc

AU - Yoon, Jinha

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AU - Lee, So Yeong

AU - Choo, Jaebum

AU - Lee, Kangtaek

AU - Joo, Sang Woo

N1 - Funding Information: We would like to thank Semi Kim for her help on TEM measurements. SWJ, Prof. So Yeong Lee, and KL thank Dr. Kyong-Ah Yoon for their helpful comments on the EGFR mutations. Prof. K. Cho acknowledges the support by the Human Resources Development of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government Ministry of Trade, Industry & Energy (No. 20124030200070 ). Prof. D. Kim acknowledges support from the Korea government (MEST) No. 2011-0031496 . Prof. J. Choo acknowledges that the Nano Material Technology Development Program supported this work through the National Research Foundation of Korea, funded by the Ministry of Education, Science, and Technology (grant number 2012035286 ). Publisher Copyright: © 2014 Elsevier B.V.

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N2 - Gefitinib (GF) is a US Food and Drug Administration-approved epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor for treating the lung cancers. We fabricated colloidal gold nanoparticle (AuNP) conjugates of the GF anticancer drug by self-assembly to test their potency against A549, NCI-H460, and NCI-H1975 lung cancer cells. GF adsorption on AuNP surfaces was examined by UV-vis absorption spectra and surface-enhanced Raman scattering. Density functional theory calculations were performed to estimate the energetic stabilities of the drug-AuNP composites. The N1 nitrogen atom of the quinazoline ring of GF was calculated to be more stable than the N3 in binding Au cluster atoms. The internalizations of GF-coated AuNPs were examined by transmission electron and dark-field microscopy. A cell viability test of AuNP-GF conjugates with the EGFR antibody exhibited much higher reductions than free GF for A549, NCI-H460, and NCI-H1975 lung cancer cells after treatment for 48. h.

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Colloidal gold nanoparticle conjugates of gefitinib

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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