Collagen XVIII and corneal reinnervation following keratectomy

Tohru Sakimoto; Tae im Kim; David Ellenberg; Naomi Fukai; Sandeep Jain; Dimitri T. Azar; Jin Hong Chang

doi:10.1016/j.febslet.2008.09.052

Collagen XVIII and corneal reinnervation following keratectomy

Tohru Sakimoto, Tae im Kim, David Ellenberg, Naomi Fukai, Sandeep Jain, Dimitri T. Azar, Jin Hong Chang

Department of Ophthalmology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The significance of collagen XVIII in the regulation of corneal reinnervation remains largely unknown. We used whole-mount immunoconfocal microscopy to localize collagen XVIII to the nerve basement membrane of wild-type (WT) mouse corneas. Transmission electron microscopy showed corneal nerve disorganization in collagen XVIII knockout mice (col18a1^-/-). Antibody 2H3-specific neurofilament colocalized with collagens XVIII and IV and laminin-2 in WT mouse corneas, but did not colocalize with collagen IV and laminin-2 in col18a1^-/- mouse corneas. Following keratectomy, col18a1^-/- mice displayed decreased corneal neurite extension compared to WT mice. Our data indicate that collagen XVIII may play an important role in corneal reinnervation after wounding.

Original language	English
Pages (from-to)	3674-3680
Number of pages	7
Journal	FEBS Letters
Volume	582
Issue number	25-26
DOIs	https://doi.org/10.1016/j.febslet.2008.09.052
Publication status	Published - 2008 Oct 29

Bibliographical note

Funding Information:
We thank Dr. Bjorn Olsen for providing col18a1 −/− mice and Patricia Pearson for TEM imaging. Supported by NIH EY14048 (J.H.C.), EY10101 (D.T.A.), EY 001792 (D.T.A.), an unrestricted departmental grant from Research to Prevent Blindness, the Bausch Lomb Japan/MEEI Research Fellowship Award, and the Japan Eye Bank Association Overseas Research Award (T.S.).

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2008.09.052

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title = "Collagen XVIII and corneal reinnervation following keratectomy",

abstract = "The significance of collagen XVIII in the regulation of corneal reinnervation remains largely unknown. We used whole-mount immunoconfocal microscopy to localize collagen XVIII to the nerve basement membrane of wild-type (WT) mouse corneas. Transmission electron microscopy showed corneal nerve disorganization in collagen XVIII knockout mice (col18a1-/-). Antibody 2H3-specific neurofilament colocalized with collagens XVIII and IV and laminin-2 in WT mouse corneas, but did not colocalize with collagen IV and laminin-2 in col18a1-/- mouse corneas. Following keratectomy, col18a1-/- mice displayed decreased corneal neurite extension compared to WT mice. Our data indicate that collagen XVIII may play an important role in corneal reinnervation after wounding.",

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AU - Ellenberg, David

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AU - Jain, Sandeep

AU - Azar, Dimitri T.

AU - Chang, Jin Hong

N1 - Funding Information: We thank Dr. Bjorn Olsen for providing col18a1 −/− mice and Patricia Pearson for TEM imaging. Supported by NIH EY14048 (J.H.C.), EY10101 (D.T.A.), EY 001792 (D.T.A.), an unrestricted departmental grant from Research to Prevent Blindness, the Bausch Lomb Japan/MEEI Research Fellowship Award, and the Japan Eye Bank Association Overseas Research Award (T.S.).

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N2 - The significance of collagen XVIII in the regulation of corneal reinnervation remains largely unknown. We used whole-mount immunoconfocal microscopy to localize collagen XVIII to the nerve basement membrane of wild-type (WT) mouse corneas. Transmission electron microscopy showed corneal nerve disorganization in collagen XVIII knockout mice (col18a1-/-). Antibody 2H3-specific neurofilament colocalized with collagens XVIII and IV and laminin-2 in WT mouse corneas, but did not colocalize with collagen IV and laminin-2 in col18a1-/- mouse corneas. Following keratectomy, col18a1-/- mice displayed decreased corneal neurite extension compared to WT mice. Our data indicate that collagen XVIII may play an important role in corneal reinnervation after wounding.

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Collagen XVIII and corneal reinnervation following keratectomy

Abstract

Bibliographical note

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