Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction

Zhong Min Che; Tae Heob Jung; Jung Hee Choi; Do Jun Yoon; Hyun Jeong Jeong; Eun Ju Lee; Jin Kim

doi:10.1016/j.bbrc.2006.05.111

Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction

Zhong Min Che, Tae Heob Jung, Jung Hee Choi, Do Jun Yoon, Hyun Jeong Jeong, Eun Ju Lee, Jin Kim

Department of Oral Pathology

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

The roles of tumor stroma in carcinogenesis are still unclear. This study was aimed at designing an in vitro model for investigating the effects of stromal fibroblasts in the invasive growth of squamous cell carcinoma. Using two cancer cell lines, we performed three-dimensional co-culture with dermal equivalents to evaluate the effects of fibroblasts in cancer invasion. In vitro models for cellular interaction study were designed as follows: a collagen gel-based direct co-culture model (C-Dr) and a collagen gel-based indirect co-culture model (C-In). The invasive growth was found only in the dermal equivalents with fibroblasts. MMP-2 activity could be induced by direct contact between cancer cells and stromal fibroblasts. Cathepsin D was also highly expressed when co-cultured with cancer cells and fibroblasts. The present study demonstrated that the presence of fibroblasts is essential in cancer invasion and that collagen gel-based co-culture models might be useful for invasive study.

Original language	English
Pages (from-to)	268-275
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	346
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2006.05.111
Publication status	Published - 2006 Jul 21

Bibliographical note

Funding Information:
This study was supported by the Korea Science and Engineering Foundation (R01-2002-000-00454-0). The authors express sincere gratitude to Ms. Linda Kilpatrick-Lee, Yonsei University, for critically reading the manuscript.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2006.05.111

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title = "Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction",

abstract = "The roles of tumor stroma in carcinogenesis are still unclear. This study was aimed at designing an in vitro model for investigating the effects of stromal fibroblasts in the invasive growth of squamous cell carcinoma. Using two cancer cell lines, we performed three-dimensional co-culture with dermal equivalents to evaluate the effects of fibroblasts in cancer invasion. In vitro models for cellular interaction study were designed as follows: a collagen gel-based direct co-culture model (C-Dr) and a collagen gel-based indirect co-culture model (C-In). The invasive growth was found only in the dermal equivalents with fibroblasts. MMP-2 activity could be induced by direct contact between cancer cells and stromal fibroblasts. Cathepsin D was also highly expressed when co-cultured with cancer cells and fibroblasts. The present study demonstrated that the presence of fibroblasts is essential in cancer invasion and that collagen gel-based co-culture models might be useful for invasive study.",

author = "Che, {Zhong Min} and Jung, {Tae Heob} and Choi, {Jung Hee} and Yoon, {Do Jun} and Jeong, {Hyun Jeong} and Lee, {Eun Ju} and Jin Kim",

note = "Funding Information: This study was supported by the Korea Science and Engineering Foundation (R01-2002-000-00454-0). The authors express sincere gratitude to Ms. Linda Kilpatrick-Lee, Yonsei University, for critically reading the manuscript.",

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AU - Jeong, Hyun Jeong

AU - Lee, Eun Ju

AU - Kim, Jin

N1 - Funding Information: This study was supported by the Korea Science and Engineering Foundation (R01-2002-000-00454-0). The authors express sincere gratitude to Ms. Linda Kilpatrick-Lee, Yonsei University, for critically reading the manuscript.

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N2 - The roles of tumor stroma in carcinogenesis are still unclear. This study was aimed at designing an in vitro model for investigating the effects of stromal fibroblasts in the invasive growth of squamous cell carcinoma. Using two cancer cell lines, we performed three-dimensional co-culture with dermal equivalents to evaluate the effects of fibroblasts in cancer invasion. In vitro models for cellular interaction study were designed as follows: a collagen gel-based direct co-culture model (C-Dr) and a collagen gel-based indirect co-culture model (C-In). The invasive growth was found only in the dermal equivalents with fibroblasts. MMP-2 activity could be induced by direct contact between cancer cells and stromal fibroblasts. Cathepsin D was also highly expressed when co-cultured with cancer cells and fibroblasts. The present study demonstrated that the presence of fibroblasts is essential in cancer invasion and that collagen gel-based co-culture models might be useful for invasive study.

AB - The roles of tumor stroma in carcinogenesis are still unclear. This study was aimed at designing an in vitro model for investigating the effects of stromal fibroblasts in the invasive growth of squamous cell carcinoma. Using two cancer cell lines, we performed three-dimensional co-culture with dermal equivalents to evaluate the effects of fibroblasts in cancer invasion. In vitro models for cellular interaction study were designed as follows: a collagen gel-based direct co-culture model (C-Dr) and a collagen gel-based indirect co-culture model (C-In). The invasive growth was found only in the dermal equivalents with fibroblasts. MMP-2 activity could be induced by direct contact between cancer cells and stromal fibroblasts. Cathepsin D was also highly expressed when co-cultured with cancer cells and fibroblasts. The present study demonstrated that the presence of fibroblasts is essential in cancer invasion and that collagen gel-based co-culture models might be useful for invasive study.

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Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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