Clustered Regularly Interspaced Short Palindromic Repeats-Mediated Surface-Enhanced Raman Scattering Assay for Multidrug-Resistant Bacteria

Hongki Kim; Soohyun Lee; Hwi Won Seo; Byunghoon Kang; Jeong Moon; Kyoung G. Lee; Dongeun Yong; Hyunju Kang; Juyeon Jung; Eun Kyung Lim; Jinyoung Jeong; Hyun Gyu Park; Choong Min Ryu; Taejoon Kang

doi:10.1021/acsnano.0c07264

Clustered Regularly Interspaced Short Palindromic Repeats-Mediated Surface-Enhanced Raman Scattering Assay for Multidrug-Resistant Bacteria

Hongki Kim, Soohyun Lee, Hwi Won Seo, Byunghoon Kang, Jeong Moon, Kyoung G. Lee, Dongeun Yong, Hyunju Kang, Juyeon Jung, Eun Kyung Lim, Jinyoung Jeong, Hyun Gyu Park, Choong Min Ryu, Taejoon Kang

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

Antimicrobial resistance and multidrug resistance are slower-moving pandemics than the fast-spreading coronavirus disease 2019; however, they have potential to cause a much greater threat to global health. Here, we report a clustered regularly interspaced short palindromic repeats (CRISPR)-mediated surface-enhanced Raman scattering (SERS) assay for multidrug-resistant (MDR) bacteria. This assay was developed via a synergistic combination of the specific gene-recognition ability of the CRISPR system, superb sensitivity of SERS, and simple separation property of magnetic nanoparticles. This assay detects three multidrug-resistant (MDR) bacteria, species Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae, without purification or gene amplification steps. Furthermore, MDR A. baumannii-infected mice were successfully diagnosed using the assay. Finally, we demonstrate the on-site capture and detection of MDR bacteria through a combination of the three-dimensional nanopillar array swab and CRISPR-mediated SERS assay. This method may prove effective for the accurate diagnosis of MDR bacterial pathogens, thus preventing severe infection by ensuring appropriate antibiotic treatment.

Original language	English
Journal	ACS Nano
Volume	14
Issue number	12
DOIs	https://doi.org/10.1021/acsnano.0c07264
Publication status	Published - 2020 Dec 22

Bibliographical note

Funding Information:
This research was supported by the Center for BioNano Health-Guard funded by the Ministry of Science and ICT of Korea (MSIT) as Global Frontier Project (H-GUARD_2013M3A6B2078950, H-GUARD_2014M3A6B2060507 and H-GUARD_2014M3A6B2060302), the Bio and Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by MSIT (NRF-2018M3A9E2022821), the Basic Science Research Program of NRF funded by MSIT (NRF-2019R1C1C1006867), and KRIBB Research initiative Program.

Funding Information:
This research was supported by the Center for BioNano Health-Guard funded by the Ministry of Science and ICT of Korea (MSIT) as Global Frontier Project (H-GUARD_2013M3A6B2078950, H-GUARD_2014M3A6B2060507, and H-GUARD_2014M3A6B2060302), the Bio and Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by MSIT (NRF-2018M3A9E2022821), the Basic Science Research Program of NRF funded by MSIT (NRF-2019R1C1C1006867), and KRIBB Research initiative Program.

Publisher Copyright:
© 2020 American Chemical Society.

All Science Journal Classification (ASJC) codes

Materials Science(all)
Engineering(all)
Physics and Astronomy(all)

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10.1021/acsnano.0c07264

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@article{64b9cad9706148d89bc3536252d0bd56,

title = "Clustered Regularly Interspaced Short Palindromic Repeats-Mediated Surface-Enhanced Raman Scattering Assay for Multidrug-Resistant Bacteria",

abstract = "Antimicrobial resistance and multidrug resistance are slower-moving pandemics than the fast-spreading coronavirus disease 2019; however, they have potential to cause a much greater threat to global health. Here, we report a clustered regularly interspaced short palindromic repeats (CRISPR)-mediated surface-enhanced Raman scattering (SERS) assay for multidrug-resistant (MDR) bacteria. This assay was developed via a synergistic combination of the specific gene-recognition ability of the CRISPR system, superb sensitivity of SERS, and simple separation property of magnetic nanoparticles. This assay detects three multidrug-resistant (MDR) bacteria, species Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae, without purification or gene amplification steps. Furthermore, MDR A. baumannii-infected mice were successfully diagnosed using the assay. Finally, we demonstrate the on-site capture and detection of MDR bacteria through a combination of the three-dimensional nanopillar array swab and CRISPR-mediated SERS assay. This method may prove effective for the accurate diagnosis of MDR bacterial pathogens, thus preventing severe infection by ensuring appropriate antibiotic treatment.",

author = "Hongki Kim and Soohyun Lee and Seo, {Hwi Won} and Byunghoon Kang and Jeong Moon and Lee, {Kyoung G.} and Dongeun Yong and Hyunju Kang and Juyeon Jung and Lim, {Eun Kyung} and Jinyoung Jeong and Park, {Hyun Gyu} and Ryu, {Choong Min} and Taejoon Kang",

note = "Funding Information: This research was supported by the Center for BioNano Health-Guard funded by the Ministry of Science and ICT of Korea (MSIT) as Global Frontier Project (H-GUARD_2013M3A6B2078950, H-GUARD_2014M3A6B2060507 and H-GUARD_2014M3A6B2060302), the Bio and Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by MSIT (NRF-2018M3A9E2022821), the Basic Science Research Program of NRF funded by MSIT (NRF-2019R1C1C1006867), and KRIBB Research initiative Program. Funding Information: This research was supported by the Center for BioNano Health-Guard funded by the Ministry of Science and ICT of Korea (MSIT) as Global Frontier Project (H-GUARD_2013M3A6B2078950, H-GUARD_2014M3A6B2060507, and H-GUARD_2014M3A6B2060302), the Bio and Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by MSIT (NRF-2018M3A9E2022821), the Basic Science Research Program of NRF funded by MSIT (NRF-2019R1C1C1006867), and KRIBB Research initiative Program. Publisher Copyright: {\textcopyright} 2020 American Chemical Society.",

year = "2020",

month = dec,

day = "22",

doi = "10.1021/acsnano.0c07264",

language = "English",

volume = "14",

journal = "ACS Nano",

issn = "1936-0851",

publisher = "American Chemical Society",

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T1 - Clustered Regularly Interspaced Short Palindromic Repeats-Mediated Surface-Enhanced Raman Scattering Assay for Multidrug-Resistant Bacteria

AU - Kim, Hongki

AU - Lee, Soohyun

AU - Seo, Hwi Won

AU - Kang, Byunghoon

AU - Moon, Jeong

AU - Lee, Kyoung G.

AU - Yong, Dongeun

AU - Kang, Hyunju

AU - Jung, Juyeon

AU - Lim, Eun Kyung

AU - Jeong, Jinyoung

AU - Park, Hyun Gyu

AU - Ryu, Choong Min

AU - Kang, Taejoon

N1 - Funding Information: This research was supported by the Center for BioNano Health-Guard funded by the Ministry of Science and ICT of Korea (MSIT) as Global Frontier Project (H-GUARD_2013M3A6B2078950, H-GUARD_2014M3A6B2060507 and H-GUARD_2014M3A6B2060302), the Bio and Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by MSIT (NRF-2018M3A9E2022821), the Basic Science Research Program of NRF funded by MSIT (NRF-2019R1C1C1006867), and KRIBB Research initiative Program. Funding Information: This research was supported by the Center for BioNano Health-Guard funded by the Ministry of Science and ICT of Korea (MSIT) as Global Frontier Project (H-GUARD_2013M3A6B2078950, H-GUARD_2014M3A6B2060507, and H-GUARD_2014M3A6B2060302), the Bio and Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by MSIT (NRF-2018M3A9E2022821), the Basic Science Research Program of NRF funded by MSIT (NRF-2019R1C1C1006867), and KRIBB Research initiative Program. Publisher Copyright: © 2020 American Chemical Society.

PY - 2020/12/22

Y1 - 2020/12/22

N2 - Antimicrobial resistance and multidrug resistance are slower-moving pandemics than the fast-spreading coronavirus disease 2019; however, they have potential to cause a much greater threat to global health. Here, we report a clustered regularly interspaced short palindromic repeats (CRISPR)-mediated surface-enhanced Raman scattering (SERS) assay for multidrug-resistant (MDR) bacteria. This assay was developed via a synergistic combination of the specific gene-recognition ability of the CRISPR system, superb sensitivity of SERS, and simple separation property of magnetic nanoparticles. This assay detects three multidrug-resistant (MDR) bacteria, species Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae, without purification or gene amplification steps. Furthermore, MDR A. baumannii-infected mice were successfully diagnosed using the assay. Finally, we demonstrate the on-site capture and detection of MDR bacteria through a combination of the three-dimensional nanopillar array swab and CRISPR-mediated SERS assay. This method may prove effective for the accurate diagnosis of MDR bacterial pathogens, thus preventing severe infection by ensuring appropriate antibiotic treatment.

AB - Antimicrobial resistance and multidrug resistance are slower-moving pandemics than the fast-spreading coronavirus disease 2019; however, they have potential to cause a much greater threat to global health. Here, we report a clustered regularly interspaced short palindromic repeats (CRISPR)-mediated surface-enhanced Raman scattering (SERS) assay for multidrug-resistant (MDR) bacteria. This assay was developed via a synergistic combination of the specific gene-recognition ability of the CRISPR system, superb sensitivity of SERS, and simple separation property of magnetic nanoparticles. This assay detects three multidrug-resistant (MDR) bacteria, species Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae, without purification or gene amplification steps. Furthermore, MDR A. baumannii-infected mice were successfully diagnosed using the assay. Finally, we demonstrate the on-site capture and detection of MDR bacteria through a combination of the three-dimensional nanopillar array swab and CRISPR-mediated SERS assay. This method may prove effective for the accurate diagnosis of MDR bacterial pathogens, thus preventing severe infection by ensuring appropriate antibiotic treatment.

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DO - 10.1021/acsnano.0c07264

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SN - 1936-0851

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Clustered Regularly Interspaced Short Palindromic Repeats-Mediated Surface-Enhanced Raman Scattering Assay for Multidrug-Resistant Bacteria

Abstract

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