TY - GEN
T1 - Clonal culture and chemodrug assay of heterogeneous cells (PC3 prostate carcinoma cells) using microfluidic single cell array chips
AU - Chung, Jaehoon
AU - Bersano-Begey, Tom
AU - Pienta, Ken
AU - Yoon, Euisik
PY - 2009
Y1 - 2009
N2 - We present a single cell assay microfluidic chip that can 1) load cells into a microwell array at single cell resolution with a high capturing efficiency, 2) grow them into homogeneous clonal colonies without cell migration between neighbouring microwells, and 3) supply cell culture media or reagents to each microwell from a perfusion gravity flow induced by pressure difference between inlet and outlet reservoirs. Using the fabricated chip, we successfully identified three clonal phenotypes in prostate cancer cells and observed the drug responsiveness varies in different subtypes.
AB - We present a single cell assay microfluidic chip that can 1) load cells into a microwell array at single cell resolution with a high capturing efficiency, 2) grow them into homogeneous clonal colonies without cell migration between neighbouring microwells, and 3) supply cell culture media or reagents to each microwell from a perfusion gravity flow induced by pressure difference between inlet and outlet reservoirs. Using the fabricated chip, we successfully identified three clonal phenotypes in prostate cancer cells and observed the drug responsiveness varies in different subtypes.
UR - http://www.scopus.com/inward/record.url?scp=84901771637&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901771637&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901771637
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 21
EP - 23
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -
