Clec14a is specifically expressed in endothelial cells and mediates cell to cell adhesion

Seung Sik Rho; Hyun Jung Choi; Jeong Ki Min; Heon Woo Lee; Hongryeol Park; Hyojin Park; Young Myeong Kim; Young Guen Kwon

doi:10.1016/j.bbrc.2010.11.075

Clec14a is specifically expressed in endothelial cells and mediates cell to cell adhesion

Seung Sik Rho, Hyun Jung Choi, Jeong Ki Min, Heon Woo Lee, Hongryeol Park, Hyojin Park, Young Myeong Kim, Young Guen Kwon

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41 Citations (Scopus)

Abstract

Clec14a is a member of the thrombomodulin (TM) family, but its function has not yet been determined. Here, we report that Clec14a is a plasma membrane protein of endothelial cells (ECs) expressed specifically in the vasculature of mice. Deletion mutant analysis revealed that Clec14a mediates cell-cell adhesion through its C-type lectin-like domain. Knockdown of Clec14a in ECs suppressed cell migratory activity and filopodial protrusion, and delayed formation of tube-like structures. These findings demonstrate that Clec14a is a novel EC-specific protein that appears to play a role in cell-cell adhesion and angiogenesis.

Original language	English
Pages (from-to)	103-108
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	404
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2010.11.075
Publication status	Published - 2011 Jan 7

Bibliographical note

Funding Information:
This work was supported by a National Research Laboratory Grant (20100018854) and the Korea Biotech R&D Group of Next-generation growth engine project (2010K0012733-08A0301-00310) from the Ministry of Education and Science Technology, and a grant from the Korea Health 21 R&D Project, Ministry of Health Welfare & Family Affairs (A085136), Korea.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2010.11.075

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title = "Clec14a is specifically expressed in endothelial cells and mediates cell to cell adhesion",

abstract = "Clec14a is a member of the thrombomodulin (TM) family, but its function has not yet been determined. Here, we report that Clec14a is a plasma membrane protein of endothelial cells (ECs) expressed specifically in the vasculature of mice. Deletion mutant analysis revealed that Clec14a mediates cell-cell adhesion through its C-type lectin-like domain. Knockdown of Clec14a in ECs suppressed cell migratory activity and filopodial protrusion, and delayed formation of tube-like structures. These findings demonstrate that Clec14a is a novel EC-specific protein that appears to play a role in cell-cell adhesion and angiogenesis.",

author = "Rho, {Seung Sik} and Choi, {Hyun Jung} and Min, {Jeong Ki} and Lee, {Heon Woo} and Hongryeol Park and Hyojin Park and Kim, {Young Myeong} and Kwon, {Young Guen}",

note = "Funding Information: This work was supported by a National Research Laboratory Grant (20100018854) and the Korea Biotech R&D Group of Next-generation growth engine project (2010K0012733-08A0301-00310) from the Ministry of Education and Science Technology, and a grant from the Korea Health 21 R&D Project, Ministry of Health Welfare & Family Affairs (A085136), Korea. ",

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AU - Rho, Seung Sik

AU - Choi, Hyun Jung

AU - Min, Jeong Ki

AU - Lee, Heon Woo

AU - Park, Hongryeol

AU - Park, Hyojin

AU - Kim, Young Myeong

AU - Kwon, Young Guen

N1 - Funding Information: This work was supported by a National Research Laboratory Grant (20100018854) and the Korea Biotech R&D Group of Next-generation growth engine project (2010K0012733-08A0301-00310) from the Ministry of Education and Science Technology, and a grant from the Korea Health 21 R&D Project, Ministry of Health Welfare & Family Affairs (A085136), Korea.

PY - 2011/1/7

Y1 - 2011/1/7

N2 - Clec14a is a member of the thrombomodulin (TM) family, but its function has not yet been determined. Here, we report that Clec14a is a plasma membrane protein of endothelial cells (ECs) expressed specifically in the vasculature of mice. Deletion mutant analysis revealed that Clec14a mediates cell-cell adhesion through its C-type lectin-like domain. Knockdown of Clec14a in ECs suppressed cell migratory activity and filopodial protrusion, and delayed formation of tube-like structures. These findings demonstrate that Clec14a is a novel EC-specific protein that appears to play a role in cell-cell adhesion and angiogenesis.

AB - Clec14a is a member of the thrombomodulin (TM) family, but its function has not yet been determined. Here, we report that Clec14a is a plasma membrane protein of endothelial cells (ECs) expressed specifically in the vasculature of mice. Deletion mutant analysis revealed that Clec14a mediates cell-cell adhesion through its C-type lectin-like domain. Knockdown of Clec14a in ECs suppressed cell migratory activity and filopodial protrusion, and delayed formation of tube-like structures. These findings demonstrate that Clec14a is a novel EC-specific protein that appears to play a role in cell-cell adhesion and angiogenesis.

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Clec14a is specifically expressed in endothelial cells and mediates cell to cell adhesion

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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