Characteristics of thermostable chitinase enzymes from the indonesian Bacillus sp.13.26

Purwani E. Yuli; Maggy Thenawidjaja Suhartono; Yaya Rukayadi; Jae Kwan Hwang; Yu Ryang Pyun

doi:10.1016/j.enzmictec.2004.03.017

Characteristics of thermostable chitinase enzymes from the indonesian Bacillus sp.13.26

Purwani E. Yuli, Maggy Thenawidjaja Suhartono, Yaya Rukayadi, Jae Kwan Hwang, Yu Ryang Pyun

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

A chitinase producing bacteria was screened and isolated from Tompaso hot spring in North Sulawesi Indonesia. The gram positive spore forming rod shaped bacteria was identified as Bacillus sp. 13.26 through sequence analysis of the 16SrRNA gene. When grown in medium containing 0.5% chitin at 55°C, the thermophillic bacteria optimally produced extracellular chitinase enzyme after 72 h of incubation. The enzyme was purified through ammonium sulfate fractionation, dialysis, heat treatment at 60°C for 3 h and chromatographed onto DEAE Sepharose CL-6B. The optimum temperature and pH of the 60-kD chitinase were found at 60°C and pH 7-8. The thermostable chitinase still retained the activity after incubation for 5 h incubation at 70°C. Zymogram analysis confirmed the thermostability of the enzyme ; when heated at 80°C, the enzyme was still significantly active even after 1 h of incubation.

Original language	English
Pages (from-to)	147-153
Number of pages	7
Journal	Enzyme and Microbial Technology
Volume	35
Issue number	2-3
DOIs	https://doi.org/10.1016/j.enzmictec.2004.03.017
Publication status	Published - 2004 Aug 5

Bibliographical note

Funding Information:
This research was supported by Research Grant on “exploration of chitinase enzymes from the Indonesian thermophilles” from Bioproduct Research Center, Yonsei University, Korea.

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.enzmictec.2004.03.017

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title = "Characteristics of thermostable chitinase enzymes from the indonesian Bacillus sp.13.26",

abstract = "A chitinase producing bacteria was screened and isolated from Tompaso hot spring in North Sulawesi Indonesia. The gram positive spore forming rod shaped bacteria was identified as Bacillus sp. 13.26 through sequence analysis of the 16SrRNA gene. When grown in medium containing 0.5% chitin at 55°C, the thermophillic bacteria optimally produced extracellular chitinase enzyme after 72 h of incubation. The enzyme was purified through ammonium sulfate fractionation, dialysis, heat treatment at 60°C for 3 h and chromatographed onto DEAE Sepharose CL-6B. The optimum temperature and pH of the 60-kD chitinase were found at 60°C and pH 7-8. The thermostable chitinase still retained the activity after incubation for 5 h incubation at 70°C. Zymogram analysis confirmed the thermostability of the enzyme ; when heated at 80°C, the enzyme was still significantly active even after 1 h of incubation.",

author = "Yuli, {Purwani E.} and Suhartono, {Maggy Thenawidjaja} and Yaya Rukayadi and Hwang, {Jae Kwan} and Pyun, {Yu Ryang}",

note = "Funding Information: This research was supported by Research Grant on “exploration of chitinase enzymes from the Indonesian thermophilles” from Bioproduct Research Center, Yonsei University, Korea. ",

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AU - Suhartono, Maggy Thenawidjaja

AU - Rukayadi, Yaya

AU - Hwang, Jae Kwan

AU - Pyun, Yu Ryang

N1 - Funding Information: This research was supported by Research Grant on “exploration of chitinase enzymes from the Indonesian thermophilles” from Bioproduct Research Center, Yonsei University, Korea.

PY - 2004/8/5

Y1 - 2004/8/5

N2 - A chitinase producing bacteria was screened and isolated from Tompaso hot spring in North Sulawesi Indonesia. The gram positive spore forming rod shaped bacteria was identified as Bacillus sp. 13.26 through sequence analysis of the 16SrRNA gene. When grown in medium containing 0.5% chitin at 55°C, the thermophillic bacteria optimally produced extracellular chitinase enzyme after 72 h of incubation. The enzyme was purified through ammonium sulfate fractionation, dialysis, heat treatment at 60°C for 3 h and chromatographed onto DEAE Sepharose CL-6B. The optimum temperature and pH of the 60-kD chitinase were found at 60°C and pH 7-8. The thermostable chitinase still retained the activity after incubation for 5 h incubation at 70°C. Zymogram analysis confirmed the thermostability of the enzyme ; when heated at 80°C, the enzyme was still significantly active even after 1 h of incubation.

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Characteristics of thermostable chitinase enzymes from the indonesian Bacillus sp.13.26

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