Chapter 22 Microscopic Technique for the Detection of Nitric Oxide-Dependent Angiogenesis in an Animal Model

Seung Namkoong, Byoung Hee Chung, Kwon Soo Ha, Hansoo Lee, Young Guen Kwon, Young Myeong Kim

Research output: Chapter in Book/Report/Conference proceedingChapter

15 Citations (Scopus)


Nitric oxide (NO) plays an important role in maintaining vascular homeostasis. The importance of NO in the vasculature is demonstrated by several experimental conditions, such as vascular endothelial growth factor (VEGF)-induced angiogenesis. Thus, the NO metabolic pathway in endothelial cells could be one of the main contributing factors for angiogenesis. Although several methods have been used for measuring in vitro angiogenesis, a proper technique has not been developed for identifying in vivo NO-dependent angiogenesis. This chapter provides a new intravital microscopic method for detecting and measuring NO-dependent angiogenesis in a mouse model. This technique showed strong abdominal neovascularization in wild-type mice, but not eNOS knockout mice, locally injected with VEGF, as well as stimulation of angiogenesis in NO donor-injected mice. This technique also revealed the inhibitory effect of the NOS inhibitor NG-iminoethyl-L-ornithine in VEGF-mediated in vivo angiogenesis. This chapter describes intravital microscopy as a new imaging technique for detecting NO-dependent angiogenesis in an animal model.

Original languageEnglish
Title of host publicationNitric Oxide, Part G Oxidative and Nitrosative Stress in Redox Regulation of Cell Signaling
PublisherAcademic Press Inc.
Number of pages10
ISBN (Print)9780123743091
Publication statusPublished - 2008

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879

Bibliographical note

Funding Information:
This work was supported by a Vascular System Research Center Grant from the Korea Science and Engineering Foundation.

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology


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