Changes in the biological characteristics of glioma cancer stem cells after serial in vivo subtransplantation

Ga Yeong Shin, Jin Kyoung Shim, Ji Hyun Lee, Hye Jin Shin, Su Jae Lee, Yong Min Huh, Eui Hyun Kim, Eun Kyung Park, Se Hoon Kim, Jong Hee Chang, Dong Seok Kim, Yong Kil Hong, Sun Ho Kim, Seok Gu Kang, Frederick F. Lang

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Purpose: Currently, the interaction between the niche and glioma cancer stem cells (gCSCs) is gaining attention. However, there are few studies concerned with the effects of repeated exposure to a new microenvironment on gCSCs characteristics. In this study, serial in vivo subtransplantation was performed to create a new microenvironment. We evaluated and compared the biological characteristics of gCSCs after serial in vivo subtransplantation. Methods: We cultured gCSCs from human glioma specimens according to cultured gliomasphere methods. The isolated gCSCs were termed zero-generation gCSCs (G0-gCSCs). By subsequent serial subtransplantation, we obtained first-generation gCSCs (G1-gCSCs) and second-generation gCSCs (G2-gCSCs). We evaluated and compared the biological characteristics of G0-gCSCs, G1-gCSCs, and G2-gCSCs. The in vitro characteristics included the morphology, surface marker profiles, and neural differentiation capacity and the in vivo characteristics was the survival of mice xenografts. Additionally, brain sections were analyzed using PCNA, TUNEL, and CD31 staining. Results: We observed no significant differences in the in vitro characteristics of G0-gCSCs, G1-gCSCs, and G2-gCSCs. However, the survival time of mice glioma xenografts was significantly decreased upon serial subtransplantation. In addition, immunohistochemical analyses showed that the number of TUNEL+ cells was significantly decreased while the number of CD31+ cells was significantly increased with serial in vivo subtransplantation. Conclusions: There were significant in vivo biological changes in gCSCs upon serial in vivo subtransplantation, which were shorter xenograft survival, increased angiogenesis, and decreased apoptosis. This study suggests that the repeated exposure to new microenvironments may affect the biological changes in gCSCs in vivo.

Original languageEnglish
Pages (from-to)55-64
Number of pages10
JournalChild's Nervous System
Issue number1
Publication statusPublished - 2013 Jan

Bibliographical note

Funding Information:
Acknowledgments This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0004506) and a grant from the National R&D Program for Cancer Control, Ministry for Health, Welfare and Family Affairs, Republic of Korea (1020340).

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Clinical Neurology


Dive into the research topics of 'Changes in the biological characteristics of glioma cancer stem cells after serial in vivo subtransplantation'. Together they form a unique fingerprint.

Cite this