Cellular stress-related protein expression in Helicobacter pylori-infected gastric epithelial AGS cells

Joo Weon Lim, Hyeyoung Kim, Jung Mogg Kim, Joo Sung Kim, Hyun Chae Jung, Kyung Hwan Kim

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21 Citations (Scopus)


Helicobacter pylori infection leads to gastroduodenal inflammation, peptic ulceration, and gastric carcinoma. Moreover, H. pylori may induce disease-specific protein expression in gastric epithelial cells. The present study was aimed at determining differentially expressed proteins in H. pylori-infected gastric epithelial AGS cells. AGS cells were treated with H. pylori at a bacterium/cell ratio of 300:1 for 12 h. Altered protein patterns as separated by two-dimensional electrophoresis using pH gradients of 4-7 were conclusively identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis of the peptide digests. Four differentially expressed proteins, whose expression levels were increased by more than two-fold in H. pylori-infected cells, were analyzed. These proteins (14-3-3 protein α/β, cullin homolog 3, α-enolase, ezrin) are known to be related to cell proliferation, cell adhesion, and carcinogenesis, and may be mediated by cellular stress, such as reactive oxygen species. In conclusion, the identification of these differentially expressed proteins provide valuable information for the understanding of the pathophysiologic mechanisms of H. pylori-induced gastric diseases, and may be useful as prognostic indices of H. pylori-related gastric disorders.

Original languageEnglish
Pages (from-to)1624-1634
Number of pages11
JournalInternational Journal of Biochemistry and Cell Biology
Issue number8
Publication statusPublished - 2004 Aug

Bibliographical note

Funding Information:
This study was supported by Grant 02-PJ1-PG3-21499-0006 from the Korean Ministry of Health and Welfare (to K.H. Kim).

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology


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