Cell-mimetic biosensors to detect avian influenza virus via viral fusion

Geunseon Park, Jong Woo Lim, Chaewon Park, Minjoo Yeom, Sojeong Lee, Kwang Soo Lyoo, Daesub Song, Seungjoo Haam

Research output: Contribution to journalArticlepeer-review


Avian influenza virus (AIV) causes acute infectious diseases in poultry, critically impacting food supply. Highly pathogenic avian influenza viruses (HPAIVs), in particular, cause morbidity and mortality, resulting in significant economic losses in the poultry industry. To prevent the spread of HPAIVs, detection at early stages is critical to implement effective countermeasures such as quarantine and isolation. Through a viral fusion mechanism, cell-mimetic nanoparticles (CMPs), developed in the current study, can rapidly detect HPAIV and low pathogenic AIV (LPAIV). The CMPs comprise polymeric nanoparticles, which are constructed using sialic acid and fluorescence resonance energy transfer (FRET) dye pairs that expose the FRET off signal in response to LPAIV and HPAIV, after activation by enzymatic cleavage in the endosomal environment. The CMPs detect a wide variety of LPAIVs and HPAIVs in biological environments. Additionally, the cross-reactivity of CMPs is determined by testing their function with different viral species. Therefore, these findings demonstrate the significant potential of the proposed strategy for mimicking viral infection in vitro and using them as a highly effective diagnostic assay to rapidly detect LPAIV and HPAIV, preventing economic losses associated with viral outbreaks.

Original languageEnglish
Article number114407
JournalBiosensors and Bioelectronics
Publication statusPublished - 2022 Sept 15

Bibliographical note

Funding Information:
This research was supported by grants from the Korea Mouse Phenotyping Project ( NRF-2019M3A9D5A01102797 ) of the Ministry of Science and ICT through the National Research Foundation (NRF) . S. Haam acknowledges support from the Korea Environment Industry & Technology Institute (KEITI) through the Technology Development Project for Biological Hazards Management in Indoor Air Project, funded by Korea Ministry of Environment (MOE) ( RE202101004 ). This work was also funded by Bio & Medical Technology Development Program of the National Research Foundation through the Ministry of Science & ICT ( NRF-2018M3A9E2022819 ).

Publisher Copyright:
© 2022 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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