TY - JOUR
T1 - Cell immobilization on polymer by air atmospheric pressure plasma jet treatment
AU - Lee, Jung Hwan
AU - Kwon, Jae Sung
AU - Om, Ji Yeon
AU - Kim, Yong Hee
AU - Choi, Eun Ha
AU - Kim, Kwang Mahn
AU - Kim, Kyoung Nam
PY - 2014/8
Y1 - 2014/8
N2 - The study of cell immobilization on delicate polymer by an air atmospheric pressure plasma jet (AAPPJ) is required for its medical application. The aim of this study was to evaluate whether AAPPJ treatment induce cell immobilization effect on delicate polymers without significant change of surface roughness by AAPPJ treatment. After surface roughness, dynamic contact angle, and chemical characteristics were investigated, the immobilization effect was evaluated with the mouse fibroblast L929 cell line. Surface roughness change was not observed (P > 0.05) in either delicate dental wax or polystyrene plate (PSP) as advancing and receding contact angles significantly decreased (P < 0.05), thanks to decreased hydrocarbon and formation of oxygen-related functional groups in treated PSP. Adherent L929 cells with elongated morphology were found in treated PSP along with the formation of immobilization markers vinculin and actin cytoskeleton. Increased PTK2 gene expression upregulated these markers on treated PSP.
AB - The study of cell immobilization on delicate polymer by an air atmospheric pressure plasma jet (AAPPJ) is required for its medical application. The aim of this study was to evaluate whether AAPPJ treatment induce cell immobilization effect on delicate polymers without significant change of surface roughness by AAPPJ treatment. After surface roughness, dynamic contact angle, and chemical characteristics were investigated, the immobilization effect was evaluated with the mouse fibroblast L929 cell line. Surface roughness change was not observed (P > 0.05) in either delicate dental wax or polystyrene plate (PSP) as advancing and receding contact angles significantly decreased (P < 0.05), thanks to decreased hydrocarbon and formation of oxygen-related functional groups in treated PSP. Adherent L929 cells with elongated morphology were found in treated PSP along with the formation of immobilization markers vinculin and actin cytoskeleton. Increased PTK2 gene expression upregulated these markers on treated PSP.
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U2 - 10.7567/JJAP.53.086202
DO - 10.7567/JJAP.53.086202
M3 - Article
AN - SCOPUS:84905962569
SN - 0021-4922
VL - 53
JO - Japanese journal of applied physics
JF - Japanese journal of applied physics
IS - 8
M1 - 086202
ER -