Carbon nanotube-based biosensor for detection of matrix metallopeptidase-9 and S-100B

Hyoung Sub Lee; Je Seung Oh; Young Wook Chang; Yoon Jung Park; Jeon Soo Shin; Kyung Hwa Yoo

doi:10.1016/j.cap.2009.07.003

Carbon nanotube-based biosensor for detection of matrix metallopeptidase-9 and S-100B

Hyoung Sub Lee, Je Seung Oh, Young Wook Chang, Yoon Jung Park, Jeon Soo Shin, Kyung Hwa Yoo

Department of Physics

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We fabricated carbon nanotube (CNT)-based biosensor with field effect transistor structure for detection of matrix metallopeptidase-9 (MMP-9) and S-100B. To minimize contact effects, the metal electrodes were passivated with SiO_x film, and anti-MMP-9 and S-100B antibody were immobilized on the CNT surface using a chemical linker. When MMP-9 or S-100B antigen was added to bind anti-MMP-9 or S-100B antibody, the decrease in electrical conductance was observed. We have also investigated the dependence of MMP-9 and S-100B concentration on the conductance change. A linear relationship was found between the conductance and the concentration in semi-logarithm scale.

Original language	English
Pages (from-to)	e270-e272
Journal	Current Applied Physics
Volume	9
Issue number	4 SUPPL.
DOIs	https://doi.org/10.1016/j.cap.2009.07.003
Publication status	Published - 2009 Jul

Bibliographical note

Funding Information:
This work has been financially supported by Korea Science and Engineering Foundation through National Core Research Center for Nanomedical Technology (Grand No. R15-20040924-00000-0) and by BK21 Project.

All Science Journal Classification (ASJC) codes

Materials Science(all)
Physics and Astronomy(all)

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Cite this

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title = "Carbon nanotube-based biosensor for detection of matrix metallopeptidase-9 and S-100B",

abstract = "We fabricated carbon nanotube (CNT)-based biosensor with field effect transistor structure for detection of matrix metallopeptidase-9 (MMP-9) and S-100B. To minimize contact effects, the metal electrodes were passivated with SiOx film, and anti-MMP-9 and S-100B antibody were immobilized on the CNT surface using a chemical linker. When MMP-9 or S-100B antigen was added to bind anti-MMP-9 or S-100B antibody, the decrease in electrical conductance was observed. We have also investigated the dependence of MMP-9 and S-100B concentration on the conductance change. A linear relationship was found between the conductance and the concentration in semi-logarithm scale.",

author = "Lee, {Hyoung Sub} and Oh, {Je Seung} and Chang, {Young Wook} and Park, {Yoon Jung} and Shin, {Jeon Soo} and Yoo, {Kyung Hwa}",

note = "Funding Information: This work has been financially supported by Korea Science and Engineering Foundation through National Core Research Center for Nanomedical Technology (Grand No. R15-20040924-00000-0) and by BK21 Project.",

year = "2009",

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doi = "10.1016/j.cap.2009.07.003",

language = "English",

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journal = "Current Applied Physics",

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AU - Lee, Hyoung Sub

AU - Oh, Je Seung

AU - Chang, Young Wook

AU - Park, Yoon Jung

AU - Shin, Jeon Soo

AU - Yoo, Kyung Hwa

N1 - Funding Information: This work has been financially supported by Korea Science and Engineering Foundation through National Core Research Center for Nanomedical Technology (Grand No. R15-20040924-00000-0) and by BK21 Project.

PY - 2009/7

Y1 - 2009/7

N2 - We fabricated carbon nanotube (CNT)-based biosensor with field effect transistor structure for detection of matrix metallopeptidase-9 (MMP-9) and S-100B. To minimize contact effects, the metal electrodes were passivated with SiOx film, and anti-MMP-9 and S-100B antibody were immobilized on the CNT surface using a chemical linker. When MMP-9 or S-100B antigen was added to bind anti-MMP-9 or S-100B antibody, the decrease in electrical conductance was observed. We have also investigated the dependence of MMP-9 and S-100B concentration on the conductance change. A linear relationship was found between the conductance and the concentration in semi-logarithm scale.

AB - We fabricated carbon nanotube (CNT)-based biosensor with field effect transistor structure for detection of matrix metallopeptidase-9 (MMP-9) and S-100B. To minimize contact effects, the metal electrodes were passivated with SiOx film, and anti-MMP-9 and S-100B antibody were immobilized on the CNT surface using a chemical linker. When MMP-9 or S-100B antigen was added to bind anti-MMP-9 or S-100B antibody, the decrease in electrical conductance was observed. We have also investigated the dependence of MMP-9 and S-100B concentration on the conductance change. A linear relationship was found between the conductance and the concentration in semi-logarithm scale.

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SP - e270-e272

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Carbon nanotube-based biosensor for detection of matrix metallopeptidase-9 and S-100B

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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