Carbohydrate microarrays containing glycosylated fluorescent probes for assessment of glycosidase activities

Ji Young Hyun; Na Rae Kang; Injae Shin

doi:10.1021/acs.orglett.8b00180

Carbohydrate microarrays containing glycosylated fluorescent probes for assessment of glycosidase activities

Ji Young Hyun, Na Rae Kang, Injae Shin

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Carbohydrate microarrays, containing glycosylated fluorescent probes, have been constructed using N-hydroxysuccinimide (NHS) ester-conjugated BSA modified surfaces. When the carbohydrate moieties were cleaved from the fluorescent probes in the conjugates by glycosidases, the fluorescence signals of the probes were enhanced. In this study, we have applied these microarrays to profile glycosidase activities and have employed them to determine IC₅₀ values of glycosidase inhibitors.

Original language	English
Pages (from-to)	1240-1243
Number of pages	4
Journal	Organic Letters
Volume	20
Issue number	4
DOIs	https://doi.org/10.1021/acs.orglett.8b00180
Publication status	Published - 2018 Feb 16

Bibliographical note

Publisher Copyright:
© 2018 American Chemical Society.

All Science Journal Classification (ASJC) codes

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1021/acs.orglett.8b00180

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AB - Carbohydrate microarrays, containing glycosylated fluorescent probes, have been constructed using N-hydroxysuccinimide (NHS) ester-conjugated BSA modified surfaces. When the carbohydrate moieties were cleaved from the fluorescent probes in the conjugates by glycosidases, the fluorescence signals of the probes were enhanced. In this study, we have applied these microarrays to profile glycosidase activities and have employed them to determine IC50 values of glycosidase inhibitors.

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Carbohydrate microarrays containing glycosylated fluorescent probes for assessment of glycosidase activities

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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