We present a direct, rapid and chemical-free detection method for hemoglobin concentration ([Hb]), based on photothermal angular light scattering. The iron oxides contained in hemoglobin molecules exhibit high absorption of 532-nm light and generate heat under the illumination of 532-nm light, which subsequently alters the refractive index of blood. We measured this photothermal change in refractive index by employing angular light scattering spectroscopy with the goal of quantifying [Hb] in blood samples. Highly sensitive [Hb] measurement of blood samples was performed by monitoring the shifts in angularly dispersed scattering patterns from the blood-loaded microcapillary tubes. Our system measured [Hb] over the range of 0.35-17.9. g/dL with a detection limit of ~0.12. g/dL. Our sensor was characterized by excellent correlation with a reference hematology analyzer (. r>0.96), and yielded a precision of 0.63. g/dL for a blood sample of 9.0. g/dL.
Bibliographical noteFunding Information:
This research was supported by the research programs of the National Research Foundation of Korea (NRF) (NRF- 2015R1A1A1A05001548 and NRF- 2010-0020790 ) and by the Korean Health Technology Research and Development Project of the Ministry for Health, Welfare and Family Affairs ( HI10C19110300 ).
© 2015 Elsevier B.V..
All Science Journal Classification (ASJC) codes
- Biomedical Engineering