Capillary-scale direct measurement of hemoglobin concentration of erythrocytes using photothermal angular light scattering

Uihan Kim, Jaewoo Song, Donghak Lee, Suho Ryu, Soocheol Kim, Jaehyun Hwang, Chulmin Joo

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9 Citations (Scopus)


We present a direct, rapid and chemical-free detection method for hemoglobin concentration ([Hb]), based on photothermal angular light scattering. The iron oxides contained in hemoglobin molecules exhibit high absorption of 532-nm light and generate heat under the illumination of 532-nm light, which subsequently alters the refractive index of blood. We measured this photothermal change in refractive index by employing angular light scattering spectroscopy with the goal of quantifying [Hb] in blood samples. Highly sensitive [Hb] measurement of blood samples was performed by monitoring the shifts in angularly dispersed scattering patterns from the blood-loaded microcapillary tubes. Our system measured [Hb] over the range of 0.35-17.9. g/dL with a detection limit of ~0.12. g/dL. Our sensor was characterized by excellent correlation with a reference hematology analyzer (. r>0.96), and yielded a precision of 0.63. g/dL for a blood sample of 9.0. g/dL.

Original languageEnglish
Pages (from-to)469-475
Number of pages7
JournalBiosensors and Bioelectronics
Publication statusPublished - 2015 Dec 5

Bibliographical note

Funding Information:
This research was supported by the research programs of the National Research Foundation of Korea (NRF) (NRF- 2015R1A1A1A05001548 and NRF- 2010-0020790 ) and by the Korean Health Technology Research and Development Project of the Ministry for Health, Welfare and Family Affairs ( HI10C19110300 ).

Publisher Copyright:
© 2015 Elsevier B.V..

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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