Biological characterization of epigallocatechin gallate complex with different steviol glucosides

Thi Thanh Hanh Nguyen, Nahyun M. Kim, Su Cheong Yeom, Songhee Han, So Hyung Kwak, Seong Bo Kim, Jun Seong Park, Il Kyoon Mok, Doman Kim

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Steviol glucosides (SGs) such as rubusoside (Ru), stevioside (Ste), rebaudioside A (RebA) and stevioside glucosides (SG) are herbal tea sweeteners that enhance the solubility and stability of a number of pharmaceutically important compounds. The complex of epigallocatechin gallate (EGCG) with 10% (w/v) each Ru, Ste, RebA or SG enhanced the water solubility of EGCG over 15 times to 345, 312, 341, or 320 mg/mL, respectively. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging (SC50) activities of EGCG, EGCG-Ru, EGCG-Ste, EGCG-RebA, and EGCG-SG in water were 5.88, 6.03, 6.52, 4.89, and 4.23 μg/mL, respectively. EGCGs complexed with different SGs maintained inhibitory activities against human intestinal maltase, human pancreatic α-amylase, and the growth of Streptococcus mutans, Helicobacter pylori, Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Clostridium difficile. In glucose tolerance test using C57BL/6 mice, plasma glucose levels in mice treated with EGCG or EGCG-Ste complex were decreased by 9.34%, which was 31.08% lower than those treated with maltose. The efficient and cost-effective EGCG-SGs production method might be applicable to produce water soluble bioactive nutraceuticals in large scale.

Original languageEnglish
Pages (from-to)512-517
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Issue number5
Publication statusPublished - 2017 Sept 1

Bibliographical note

Funding Information:
This work was partially supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through High Value-added Food Technology Development Program (1403-20160048) funded by Ministry of Agriculture, Food and Rural Affairs, Republic of Korea and by a grant (NRF-2015R1D1A1A01056929; D. Kim, and 2015R1D1A4A01020522; T.T. Hanh Nguyen) of the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Republic of Korea.

Publisher Copyright:
© 2017, The Korean Society for Biotechnology and Bioengineering and Springer-Verlag GmbH Germany, part of Springer Nature.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


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