Bi-compartmental 3D scaffolds for the co-culture of intervertebral disk cells and mesenchymal stem cells

Yun Min Kook, Young Mi Kang, Seong Hwan Moon, Won Gun Koh

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


The combination of electrospinning and the subsequent gelation of alginate produced bi-compartmental hydrogel consisting of a nanofiber-incorporated hydrogel matrix domain and a bare alginate hydrogel domain. The co-culture system was prepared by placing intervertebral disk (IVD) cells in the bare alginate hydrogel and human bone marrow mesenchymal stem cells (hMSCs) in the nanofiber-incorporated hydrogel. Real-time polymerase chain reaction (PCR), western blotting, immunofluorescence staining, and sulfated glycosaminoglycan assays revealed that the co-cultured groups produced more collagen type II, aggrecan, glucose transporter-1 (GLUT-1), and glycosaminoglycans (GAG) than the single-cultured hMSCs, confirming the enhanced differentiation of hMSCs in the co-culture system. It is expected that our bi-compartmental 3D scaffold can be applied to heterotypic co-culture systems for the study of various cell-cell interactions.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalJournal of Industrial and Engineering Chemistry
Publication statusPublished - 2016 Jun 25

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation (NRF) grant funded by the Ministry of Science, ICT and Future Planning (MSIP) ( 2015R1A2A1A15054532 and 2015R1D1A1A01060444 ) and by a grant ( HI15C1744 ) from the Korean Health Technology R&D Project through the Korean Health Industry Development Institute (KHIDI).

Publisher Copyright:
© 2016 The Korean Society of Industrial and Engineering Chemistry.

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)


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