Beneficial effects of Diplectria barbata (Wall. Ex C. B. Clarke) Franken et Roos extract on aging and antioxidants in vitro and in vivo

Youngeun Hong; Hyunji Lee; Quangdon Tran; Choinyam Bayarmunkh; Damdindorj Boldbaatar; So Hee Kwon; Jongsun Park; Jisoo Park

doi:10.1007/s43188-020-00064-z

Beneficial effects of Diplectria barbata (Wall. Ex C. B. Clarke) Franken et Roos extract on aging and antioxidants in vitro and in vivo

Youngeun Hong, Hyunji Lee, Quangdon Tran, Choinyam Bayarmunkh, Damdindorj Boldbaatar, So Hee Kwon, Jongsun Park, Jisoo Park

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The purpose of this study is to explore the effects of Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roons (DFR) on wound healing, antioxidant and aging in Normal Human Dermal Fibroblast cell (NHDF) cells and mouse skin models. We investigated the effects of the aging process in vitro and in vivo. DFRtreated NHDF cells showed a concentration-dependent increase in the expression of extracellular matrix (ECM) proteins (Collagen-2.5-fold increase at 50 μg/ml, Elastin-1.5-fold increase at 1μg/ml) as well as an increase in proteins related to cell survival, differentiation, and development, while expression of aging proteins such as matrix metalloproteinase 3 (MMP-3) was decreased (5-fold decrease at 50 μg/ml). DFR treatment also led to enhanced expression of antioxidant proteins such as nuclear factor erythroid 2-related factor 2 (10-fold increase at 50 μg/ml) and heme oxygenase 1 (1.5-fold increase at 25 μg/ml). To further investigate the antioxidative effects of DFR extracts, the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities were also evaluated. DFR extracts improved wound healing and resulted in increased expression of ECM proteins, while enzymes involved in collagen degradation, including MMP-3, were decreased in NHDF cells as well as in a mouse model. This study demonstrates the anti-aging, antioxidant, and wound healing properties of DFR extracts. Therefore, DFR extracts present may facilitate skin protection and care.

Original language	English
Pages (from-to)	71-83
Number of pages	13
Journal	Toxicological Research
Volume	37
Issue number	1
DOIs	https://doi.org/10.1007/s43188-020-00064-z
Publication status	Published - 2021 Jan

Bibliographical note

Funding Information:
We would like to thank KRIBB (Korea Research Institute of bioscience & Biotechnology) for providing to DFR Extracts. This work was financially supported by a research fund from Chungnam National University (grant to J. Park) and by the Brain Korea 21 PLUS Project for Medical Science, Chungnam National University School of Medicine. The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see: http://www.textcheck.com/certificate/LL0V6r.

Funding Information:
We would like to thank KRIBB (Korea Research Institute of bioscience & Biotechnology) for providing to DFR Extracts. This work was financially supported by a research fund from Chungnam National University (grant to J. Park) and by the Brain Korea 21 PLUS Project for Medical Science, Chungnam National University School of Medicine.

Publisher Copyright:
© 2020, Korean Society of Toxicology.

All Science Journal Classification (ASJC) codes

Toxicology
Health, Toxicology and Mutagenesis

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10.1007/s43188-020-00064-z

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title = "Beneficial effects of Diplectria barbata (Wall. Ex C. B. Clarke) Franken et Roos extract on aging and antioxidants in vitro and in vivo",

abstract = "The purpose of this study is to explore the effects of Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roons (DFR) on wound healing, antioxidant and aging in Normal Human Dermal Fibroblast cell (NHDF) cells and mouse skin models. We investigated the effects of the aging process in vitro and in vivo. DFRtreated NHDF cells showed a concentration-dependent increase in the expression of extracellular matrix (ECM) proteins (Collagen-2.5-fold increase at 50 μg/ml, Elastin-1.5-fold increase at 1μg/ml) as well as an increase in proteins related to cell survival, differentiation, and development, while expression of aging proteins such as matrix metalloproteinase 3 (MMP-3) was decreased (5-fold decrease at 50 μg/ml). DFR treatment also led to enhanced expression of antioxidant proteins such as nuclear factor erythroid 2-related factor 2 (10-fold increase at 50 μg/ml) and heme oxygenase 1 (1.5-fold increase at 25 μg/ml). To further investigate the antioxidative effects of DFR extracts, the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities were also evaluated. DFR extracts improved wound healing and resulted in increased expression of ECM proteins, while enzymes involved in collagen degradation, including MMP-3, were decreased in NHDF cells as well as in a mouse model. This study demonstrates the anti-aging, antioxidant, and wound healing properties of DFR extracts. Therefore, DFR extracts present may facilitate skin protection and care.",

author = "Youngeun Hong and Hyunji Lee and Quangdon Tran and Choinyam Bayarmunkh and Damdindorj Boldbaatar and Kwon, {So Hee} and Jongsun Park and Jisoo Park",

note = "Funding Information: We would like to thank KRIBB (Korea Research Institute of bioscience & Biotechnology) for providing to DFR Extracts. This work was financially supported by a research fund from Chungnam National University (grant to J. Park) and by the Brain Korea 21 PLUS Project for Medical Science, Chungnam National University School of Medicine. The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see: http://www.textcheck.com/certificate/LL0V6r. Funding Information: We would like to thank KRIBB (Korea Research Institute of bioscience & Biotechnology) for providing to DFR Extracts. This work was financially supported by a research fund from Chungnam National University (grant to J. Park) and by the Brain Korea 21 PLUS Project for Medical Science, Chungnam National University School of Medicine. Publisher Copyright: {\textcopyright} 2020, Korean Society of Toxicology.",

year = "2021",

month = jan,

doi = "10.1007/s43188-020-00064-z",

language = "English",

volume = "37",

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T1 - Beneficial effects of Diplectria barbata (Wall. Ex C. B. Clarke) Franken et Roos extract on aging and antioxidants in vitro and in vivo

AU - Hong, Youngeun

AU - Lee, Hyunji

AU - Tran, Quangdon

AU - Bayarmunkh, Choinyam

AU - Boldbaatar, Damdindorj

AU - Kwon, So Hee

AU - Park, Jongsun

AU - Park, Jisoo

N1 - Funding Information: We would like to thank KRIBB (Korea Research Institute of bioscience & Biotechnology) for providing to DFR Extracts. This work was financially supported by a research fund from Chungnam National University (grant to J. Park) and by the Brain Korea 21 PLUS Project for Medical Science, Chungnam National University School of Medicine. The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see: http://www.textcheck.com/certificate/LL0V6r. Funding Information: We would like to thank KRIBB (Korea Research Institute of bioscience & Biotechnology) for providing to DFR Extracts. This work was financially supported by a research fund from Chungnam National University (grant to J. Park) and by the Brain Korea 21 PLUS Project for Medical Science, Chungnam National University School of Medicine. Publisher Copyright: © 2020, Korean Society of Toxicology.

PY - 2021/1

Y1 - 2021/1

N2 - The purpose of this study is to explore the effects of Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roons (DFR) on wound healing, antioxidant and aging in Normal Human Dermal Fibroblast cell (NHDF) cells and mouse skin models. We investigated the effects of the aging process in vitro and in vivo. DFRtreated NHDF cells showed a concentration-dependent increase in the expression of extracellular matrix (ECM) proteins (Collagen-2.5-fold increase at 50 μg/ml, Elastin-1.5-fold increase at 1μg/ml) as well as an increase in proteins related to cell survival, differentiation, and development, while expression of aging proteins such as matrix metalloproteinase 3 (MMP-3) was decreased (5-fold decrease at 50 μg/ml). DFR treatment also led to enhanced expression of antioxidant proteins such as nuclear factor erythroid 2-related factor 2 (10-fold increase at 50 μg/ml) and heme oxygenase 1 (1.5-fold increase at 25 μg/ml). To further investigate the antioxidative effects of DFR extracts, the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities were also evaluated. DFR extracts improved wound healing and resulted in increased expression of ECM proteins, while enzymes involved in collagen degradation, including MMP-3, were decreased in NHDF cells as well as in a mouse model. This study demonstrates the anti-aging, antioxidant, and wound healing properties of DFR extracts. Therefore, DFR extracts present may facilitate skin protection and care.

AB - The purpose of this study is to explore the effects of Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roons (DFR) on wound healing, antioxidant and aging in Normal Human Dermal Fibroblast cell (NHDF) cells and mouse skin models. We investigated the effects of the aging process in vitro and in vivo. DFRtreated NHDF cells showed a concentration-dependent increase in the expression of extracellular matrix (ECM) proteins (Collagen-2.5-fold increase at 50 μg/ml, Elastin-1.5-fold increase at 1μg/ml) as well as an increase in proteins related to cell survival, differentiation, and development, while expression of aging proteins such as matrix metalloproteinase 3 (MMP-3) was decreased (5-fold decrease at 50 μg/ml). DFR treatment also led to enhanced expression of antioxidant proteins such as nuclear factor erythroid 2-related factor 2 (10-fold increase at 50 μg/ml) and heme oxygenase 1 (1.5-fold increase at 25 μg/ml). To further investigate the antioxidative effects of DFR extracts, the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities were also evaluated. DFR extracts improved wound healing and resulted in increased expression of ECM proteins, while enzymes involved in collagen degradation, including MMP-3, were decreased in NHDF cells as well as in a mouse model. This study demonstrates the anti-aging, antioxidant, and wound healing properties of DFR extracts. Therefore, DFR extracts present may facilitate skin protection and care.

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Beneficial effects of Diplectria barbata (Wall. Ex C. B. Clarke) Franken et Roos extract on aging and antioxidants in vitro and in vivo

Abstract

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