Bench-scale ex situ diesel removal process using a biobarrier and surfactant flushing

Young Chul Lee; Sung Geun Woo; Eun Sil Choi; Yeonghee Ahn; Joonhong Park; Myungjin Lee; Ji Won Yang

doi:10.1016/j.jiec.2012.01.020

Bench-scale ex situ diesel removal process using a biobarrier and surfactant flushing

Young Chul Lee, Sung Geun Woo, Eun Sil Choi, Yeonghee Ahn, Joonhong Park, Myungjin Lee, Ji Won Yang

Department of Civil and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Ex situ diesel removal was demonstrated using a biobarrier with immobilized cells and surfactant flushing in a bench-scale system. Four strains (two Acinetobacter sp., one Gordonia sp., and one Rhodococcus sp.) isolated from a diesel-contaminated site were immobilized onto a matrix to act as a biofiller. Peat moss, bentonite, and alginate were used as a hybrid support, and a procedure for the use of a bench-scale biobarrier was also employed. According to a microbial counting assay used for the biobarrier, the total amount of bacterial cells increased from approximately 2×10 ⁹ to 8×10 ⁹ (colony forming unit (CFU)/g) and the amount of inoculated diesel-degrading bacteria slightly increased from ∼2×10 ⁶ to ∼5×10 ⁶ (CFU/g) in the same period (over 30 days). This increase resulted in the reduction of diesel from 6000±45mg/kg to below 5mg/kg. While 99.9%, i.e. up to below 5mg/L of the diesel in soil was treated during 30 days of operation, diesel biodegradation accounted for 24.3% of the reduction of diesel.

Original language	English
Pages (from-to)	882-887
Number of pages	6
Journal	Journal of Industrial and Engineering Chemistry
Volume	18
Issue number	3
DOIs	https://doi.org/10.1016/j.jiec.2012.01.020
Publication status	Published - 2012 May 25

Bibliographical note

Funding Information:
This work was supported by the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Education, Science and Technology ( ABC-2010-0029728 ) and supported by the Korean Ministry of Environment as “GAIA project.” We also acknowledge the Korea Research Institute of Bioscience and Biotechnology (KRIBB) to get the bioSEM images.

All Science Journal Classification (ASJC) codes

Chemical Engineering(all)

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10.1016/j.jiec.2012.01.020

Cite this

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title = "Bench-scale ex situ diesel removal process using a biobarrier and surfactant flushing",

abstract = "Ex situ diesel removal was demonstrated using a biobarrier with immobilized cells and surfactant flushing in a bench-scale system. Four strains (two Acinetobacter sp., one Gordonia sp., and one Rhodococcus sp.) isolated from a diesel-contaminated site were immobilized onto a matrix to act as a biofiller. Peat moss, bentonite, and alginate were used as a hybrid support, and a procedure for the use of a bench-scale biobarrier was also employed. According to a microbial counting assay used for the biobarrier, the total amount of bacterial cells increased from approximately 2×10 9 to 8×10 9 (colony forming unit (CFU)/g) and the amount of inoculated diesel-degrading bacteria slightly increased from ∼2×10 6 to ∼5×10 6 (CFU/g) in the same period (over 30 days). This increase resulted in the reduction of diesel from 6000±45mg/kg to below 5mg/kg. While 99.9%, i.e. up to below 5mg/L of the diesel in soil was treated during 30 days of operation, diesel biodegradation accounted for 24.3% of the reduction of diesel.",

author = "Lee, {Young Chul} and Woo, {Sung Geun} and Choi, {Eun Sil} and Yeonghee Ahn and Joonhong Park and Myungjin Lee and Yang, {Ji Won}",

note = "Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Education, Science and Technology ( ABC-2010-0029728 ) and supported by the Korean Ministry of Environment as “GAIA project.” We also acknowledge the Korea Research Institute of Bioscience and Biotechnology (KRIBB) to get the bioSEM images. ",

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AU - Lee, Young Chul

AU - Woo, Sung Geun

AU - Choi, Eun Sil

AU - Ahn, Yeonghee

AU - Park, Joonhong

AU - Lee, Myungjin

AU - Yang, Ji Won

N1 - Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Education, Science and Technology ( ABC-2010-0029728 ) and supported by the Korean Ministry of Environment as “GAIA project.” We also acknowledge the Korea Research Institute of Bioscience and Biotechnology (KRIBB) to get the bioSEM images.

PY - 2012/5/25

Y1 - 2012/5/25

N2 - Ex situ diesel removal was demonstrated using a biobarrier with immobilized cells and surfactant flushing in a bench-scale system. Four strains (two Acinetobacter sp., one Gordonia sp., and one Rhodococcus sp.) isolated from a diesel-contaminated site were immobilized onto a matrix to act as a biofiller. Peat moss, bentonite, and alginate were used as a hybrid support, and a procedure for the use of a bench-scale biobarrier was also employed. According to a microbial counting assay used for the biobarrier, the total amount of bacterial cells increased from approximately 2×10 9 to 8×10 9 (colony forming unit (CFU)/g) and the amount of inoculated diesel-degrading bacteria slightly increased from ∼2×10 6 to ∼5×10 6 (CFU/g) in the same period (over 30 days). This increase resulted in the reduction of diesel from 6000±45mg/kg to below 5mg/kg. While 99.9%, i.e. up to below 5mg/L of the diesel in soil was treated during 30 days of operation, diesel biodegradation accounted for 24.3% of the reduction of diesel.

AB - Ex situ diesel removal was demonstrated using a biobarrier with immobilized cells and surfactant flushing in a bench-scale system. Four strains (two Acinetobacter sp., one Gordonia sp., and one Rhodococcus sp.) isolated from a diesel-contaminated site were immobilized onto a matrix to act as a biofiller. Peat moss, bentonite, and alginate were used as a hybrid support, and a procedure for the use of a bench-scale biobarrier was also employed. According to a microbial counting assay used for the biobarrier, the total amount of bacterial cells increased from approximately 2×10 9 to 8×10 9 (colony forming unit (CFU)/g) and the amount of inoculated diesel-degrading bacteria slightly increased from ∼2×10 6 to ∼5×10 6 (CFU/g) in the same period (over 30 days). This increase resulted in the reduction of diesel from 6000±45mg/kg to below 5mg/kg. While 99.9%, i.e. up to below 5mg/L of the diesel in soil was treated during 30 days of operation, diesel biodegradation accounted for 24.3% of the reduction of diesel.

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Bench-scale ex situ diesel removal process using a biobarrier and surfactant flushing

Abstract

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