Basal c-Jun N-terminal kinases promote mitotic progression through histone H3 phosphorylation

Kyunghee Lee, Kiwon Song

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Phosphorylation of histone H3 at serine 10 (S10) is essential for the onset of mitosis. Here, we show that basal c-Jun N-terminal kinases (JNKs) are required for mitotic histone H3-S10 phosphorylation in human primary fibroblast IMR90 cells. Inhibition of JNKs by specific pharmacologic inhibitors, expression of dominant-negative JNK1 and 2 mutants, or RNAi of JNK1 and 2 prevented phosphorylation of histone H3 at S10 in vivo. The JNK-specific inhibitor SP600125 blocked mitotic entry, as shown by its ability to prevent CDK1 dephosphorylation and cyclin A degradation. Basal JNK phosphorylation increased at G2/M phase, although total JNK protein levels remained unchanged. In addition, basal JNKs were localized in nuclei and centrosomes during this time, suggesting that the nuclear localization of JNKs during G2/M is tightly coupled with histone H3 phosphorylation. Basal JNKs were able to phosphorylate histone H3 in vitro and co-precipitation of histone H3 and JNKs was only detected at G2/M. Taken together, these data strongly suggest that basal JNKs play a key role in controlling histone H3 phosphorylation for mitotic entry at G2/M phase.

Original languageEnglish
Pages (from-to)216-221
Number of pages6
JournalCell Cycle
Issue number2
Publication statusPublished - 2008 Jan 15

Bibliographical note

Funding Information:
This work was supported by a grant from the National R&D Program for Cancer Control, Ministry of Health & Welfare, Republic of Korea (NCCR-0620110-1, 2007-8-0734) and a grant from the Korean Research Foundation (MOEHRD) (KRF-2006-8-1284).

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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