Avocado oil extract modulates auditory hair cell function through the regulation of amino acid biosynthesis genes

Youn Hee Nam; Isabel Rodriguez; Seo Yeon Jeong; Thu Nguyen Minh Pham; Wanlapa Nuankaew; Yun Hee Kim; Rodrigo Castañeda; Seo Yule Jeong; Min Seon Park; Kye Wan Lee; Jung Suk Lee; Do Hoon Kim; Yu Hwa Park; Seung Hyun Kim; In Seok Moon; Se Young Choung; Bin Na Hong; Kwang Won Jeong; Tong Ho Kang

doi:10.3390/nu11010113

Avocado oil extract modulates auditory hair cell function through the regulation of amino acid biosynthesis genes

Youn Hee Nam, Isabel Rodriguez, Seo Yeon Jeong, Thu Nguyen Minh Pham, Wanlapa Nuankaew, Yun Hee Kim, Rodrigo Castañeda, Seo Yule Jeong, Min Seon Park, Kye Wan Lee, Jung Suk Lee, Do Hoon Kim, Yu Hwa Park, Seung Hyun Kim, In Seok Moon, Se Young Choung, Bin Na Hong, Kwang Won Jeong, Tong Ho Kang

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Sensorineural hearing loss (SNHL) is one of the most common causes of disability, affecting over 466 million people worldwide. However, prevention or therapy of SNHL has not been widely studied. Avocado oil has shown many health benefits but it has not yet been studied in regards to SNHL. Therefore, we aimed to investigate the efficacy of avocado oil on SNHL in vitro and in vivo and elucidate its mode of action. For the present study, we used enhanced functional avocado oil extract (DKB122). DKB122 led to recovery of otic hair cells in zebrafish after neomycin-induced otic cell damage. Also, DKB122 improved auditory sensory transmission function in a mouse model of noise induced-hearing loss and protected sensory hair cells in the cochlea. In addition, RNA sequencing was performed to elucidate the mechanism involved. KEGG pathway enrichment analysis of differentially expressed genes showed that DKB122 protected House Ear Institute-Organ of Corti 1 (HEI-OC1) cells against neomycin-related alterations in gene expression due to oxidative stress, cytokine production and protein synthesis.

Original language	English
Article number	113
Journal	Nutrients
Volume	11
Issue number	1
DOIs	https://doi.org/10.3390/nu11010113
Publication status	Published - 2019 Jan

Bibliographical note

Funding Information:
Funding: This work was supported by the Technology Innovation Program (10080691, Development of an individual approval health functional food and global product; protection of hearing loss and improvement of auditory function for the people with light to moderate hearing loss (26–55 dB of pure tone audiometry threshold) using avocado fraction) funded By the Ministry of Trade, Industry & Energy (MOTIE, Korea).

Publisher Copyright:
© 2019, MDPI AG. All rights reserved.

All Science Journal Classification (ASJC) codes

Food Science
Nutrition and Dietetics

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10.3390/nu11010113

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Nam, Y. H., Rodriguez, I., Jeong, S. Y., Pham, T. N. M., Nuankaew, W., Kim, Y. H., Castañeda, R., Jeong, S. Y., Park, M. S., Lee, K. W., Lee, J. S., Kim, D. H., Park, Y. H., Kim, S. H., Moon, I. S., Choung, S. Y., Hong, B. N., Jeong, K. W., & Kang, T. H. (2019). Avocado oil extract modulates auditory hair cell function through the regulation of amino acid biosynthesis genes. Nutrients, 11(1), [113]. https://doi.org/10.3390/nu11010113

@article{ef274c54bc25415ba7ce3e6366502fd8,

title = "Avocado oil extract modulates auditory hair cell function through the regulation of amino acid biosynthesis genes",

abstract = "Sensorineural hearing loss (SNHL) is one of the most common causes of disability, affecting over 466 million people worldwide. However, prevention or therapy of SNHL has not been widely studied. Avocado oil has shown many health benefits but it has not yet been studied in regards to SNHL. Therefore, we aimed to investigate the efficacy of avocado oil on SNHL in vitro and in vivo and elucidate its mode of action. For the present study, we used enhanced functional avocado oil extract (DKB122). DKB122 led to recovery of otic hair cells in zebrafish after neomycin-induced otic cell damage. Also, DKB122 improved auditory sensory transmission function in a mouse model of noise induced-hearing loss and protected sensory hair cells in the cochlea. In addition, RNA sequencing was performed to elucidate the mechanism involved. KEGG pathway enrichment analysis of differentially expressed genes showed that DKB122 protected House Ear Institute-Organ of Corti 1 (HEI-OC1) cells against neomycin-related alterations in gene expression due to oxidative stress, cytokine production and protein synthesis.",

author = "Nam, {Youn Hee} and Isabel Rodriguez and Jeong, {Seo Yeon} and Pham, {Thu Nguyen Minh} and Wanlapa Nuankaew and Kim, {Yun Hee} and Rodrigo Casta{\~n}eda and Jeong, {Seo Yule} and Park, {Min Seon} and Lee, {Kye Wan} and Lee, {Jung Suk} and Kim, {Do Hoon} and Park, {Yu Hwa} and Kim, {Seung Hyun} and Moon, {In Seok} and Choung, {Se Young} and Hong, {Bin Na} and Jeong, {Kwang Won} and Kang, {Tong Ho}",

note = "Funding Information: Funding: This work was supported by the Technology Innovation Program (10080691, Development of an individual approval health functional food and global product; protection of hearing loss and improvement of auditory function for the people with light to moderate hearing loss (26–55 dB of pure tone audiometry threshold) using avocado fraction) funded By the Ministry of Trade, Industry & Energy (MOTIE, Korea). Publisher Copyright: {\textcopyright} 2019, MDPI AG. All rights reserved.",

year = "2019",

month = jan,

doi = "10.3390/nu11010113",

language = "English",

volume = "11",

journal = "Nutrients",

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Nam, YH, Rodriguez, I, Jeong, SY, Pham, TNM, Nuankaew, W, Kim, YH, Castañeda, R, Jeong, SY, Park, MS, Lee, KW, Lee, JS, Kim, DH, Park, YH, Kim, SH , Moon, IS, Choung, SY, Hong, BN, Jeong, KW & Kang, TH 2019, 'Avocado oil extract modulates auditory hair cell function through the regulation of amino acid biosynthesis genes', Nutrients, vol. 11, no. 1, 113. https://doi.org/10.3390/nu11010113

TY - JOUR

T1 - Avocado oil extract modulates auditory hair cell function through the regulation of amino acid biosynthesis genes

AU - Nam, Youn Hee

AU - Rodriguez, Isabel

AU - Jeong, Seo Yeon

AU - Pham, Thu Nguyen Minh

AU - Nuankaew, Wanlapa

AU - Kim, Yun Hee

AU - Castañeda, Rodrigo

AU - Jeong, Seo Yule

AU - Park, Min Seon

AU - Lee, Kye Wan

AU - Lee, Jung Suk

AU - Kim, Do Hoon

AU - Park, Yu Hwa

AU - Kim, Seung Hyun

AU - Moon, In Seok

AU - Choung, Se Young

AU - Hong, Bin Na

AU - Jeong, Kwang Won

AU - Kang, Tong Ho

N1 - Funding Information: Funding: This work was supported by the Technology Innovation Program (10080691, Development of an individual approval health functional food and global product; protection of hearing loss and improvement of auditory function for the people with light to moderate hearing loss (26–55 dB of pure tone audiometry threshold) using avocado fraction) funded By the Ministry of Trade, Industry & Energy (MOTIE, Korea). Publisher Copyright: © 2019, MDPI AG. All rights reserved.

PY - 2019/1

Y1 - 2019/1

N2 - Sensorineural hearing loss (SNHL) is one of the most common causes of disability, affecting over 466 million people worldwide. However, prevention or therapy of SNHL has not been widely studied. Avocado oil has shown many health benefits but it has not yet been studied in regards to SNHL. Therefore, we aimed to investigate the efficacy of avocado oil on SNHL in vitro and in vivo and elucidate its mode of action. For the present study, we used enhanced functional avocado oil extract (DKB122). DKB122 led to recovery of otic hair cells in zebrafish after neomycin-induced otic cell damage. Also, DKB122 improved auditory sensory transmission function in a mouse model of noise induced-hearing loss and protected sensory hair cells in the cochlea. In addition, RNA sequencing was performed to elucidate the mechanism involved. KEGG pathway enrichment analysis of differentially expressed genes showed that DKB122 protected House Ear Institute-Organ of Corti 1 (HEI-OC1) cells against neomycin-related alterations in gene expression due to oxidative stress, cytokine production and protein synthesis.

AB - Sensorineural hearing loss (SNHL) is one of the most common causes of disability, affecting over 466 million people worldwide. However, prevention or therapy of SNHL has not been widely studied. Avocado oil has shown many health benefits but it has not yet been studied in regards to SNHL. Therefore, we aimed to investigate the efficacy of avocado oil on SNHL in vitro and in vivo and elucidate its mode of action. For the present study, we used enhanced functional avocado oil extract (DKB122). DKB122 led to recovery of otic hair cells in zebrafish after neomycin-induced otic cell damage. Also, DKB122 improved auditory sensory transmission function in a mouse model of noise induced-hearing loss and protected sensory hair cells in the cochlea. In addition, RNA sequencing was performed to elucidate the mechanism involved. KEGG pathway enrichment analysis of differentially expressed genes showed that DKB122 protected House Ear Institute-Organ of Corti 1 (HEI-OC1) cells against neomycin-related alterations in gene expression due to oxidative stress, cytokine production and protein synthesis.

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ER -

Avocado oil extract modulates auditory hair cell function through the regulation of amino acid biosynthesis genes

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