Abstract
We report an automated and integrated microsystems for high-resolution imaging and high-throughput sorting of Caenorhabditis elegans (C. elegans). Microfluidic devices are developed to manipulate worms and brief cooling on-chip is used to immobilize the worms without anesthetics. High-resolution images of worms in the devices with a 100X objective are obtained and show the resolution of subcellular structures, comparable to conventional microscopy. We successfully sorted transgenic worms based on their expression patterns of fluorescence proteins with integrated Matlab algorithms that fully automate the sorting process.
| Original language | English |
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| Title of host publication | Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 |
| Editors | Jean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1219-1221 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806407 |
| Publication status | Published - 2007 |
| Event | 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France Duration: 2007 Oct 7 → 2007 Oct 11 |
Publication series
| Name | Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 |
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Conference
| Conference | 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 |
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| Country/Territory | France |
| City | Paris |
| Period | 07/10/7 → 07/10/11 |
Bibliographical note
Publisher Copyright:© 2007 CBMS.
All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)
- Bioengineering