ATG5 expression induced by MDMA (ecstasy), interferes with neuronal differentiation of neuroblastoma cells

Myounghee Chae; Gyu Seek Rhee; Ik Soon Jang; Kwangsoo Kim; Ji Hae Lee; Seung Yeul Lee; Minjung Kim; Junyoung Yang; Junsoo Park; Seung Hoon Lee

doi:10.1007/s10059-009-0075-2

ATG5 expression induced by MDMA (ecstasy), interferes with neuronal differentiation of neuroblastoma cells

Myounghee Chae, Gyu Seek Rhee, Ik Soon Jang, Kwangsoo Kim, Ji Hae Lee, Seung Yeul Lee, Minjung Kim, Junyoung Yang, Junsoo Park, Seung Hoon Lee

Division of Bioscience and Biotechnology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The amphetamine derivative 3, 4-methylenedioxymethamphetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblastoma cells after MDMA treatment. This elevated Atg5 expression interferes with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.

Original language	English
Pages (from-to)	571-575
Number of pages	5
Journal	Molecules and cells
Volume	27
Issue number	5
DOIs	https://doi.org/10.1007/s10059-009-0075-2
Publication status	Published - 2009

Bibliographical note

Funding Information:
This research was supported by a grant (07142KFDA553) from Korea Food and Drug Administration in 2007 and partly by a Korea Basic Science Institute grant (KBSI-GJ-D28026 and K-MeP).

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology

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10.1007/s10059-009-0075-2

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@article{2406b24d86134e148ae830263fd2dc8f,

title = "ATG5 expression induced by MDMA (ecstasy), interferes with neuronal differentiation of neuroblastoma cells",

abstract = "The amphetamine derivative 3, 4-methylenedioxymethamphetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblastoma cells after MDMA treatment. This elevated Atg5 expression interferes with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.",

author = "Myounghee Chae and Rhee, {Gyu Seek} and Jang, {Ik Soon} and Kwangsoo Kim and Lee, {Ji Hae} and Lee, {Seung Yeul} and Minjung Kim and Junyoung Yang and Junsoo Park and Lee, {Seung Hoon}",

note = "Funding Information: This research was supported by a grant (07142KFDA553) from Korea Food and Drug Administration in 2007 and partly by a Korea Basic Science Institute grant (KBSI-GJ-D28026 and K-MeP).",

year = "2009",

doi = "10.1007/s10059-009-0075-2",

language = "English",

volume = "27",

pages = "571--575",

journal = "Molecules and cells",

issn = "1016-8478",

publisher = "Korean Society for Molecular and Cellular Biology",

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}

TY - JOUR

T1 - ATG5 expression induced by MDMA (ecstasy), interferes with neuronal differentiation of neuroblastoma cells

AU - Chae, Myounghee

AU - Rhee, Gyu Seek

AU - Jang, Ik Soon

AU - Kim, Kwangsoo

AU - Lee, Ji Hae

AU - Lee, Seung Yeul

AU - Kim, Minjung

AU - Yang, Junyoung

AU - Park, Junsoo

AU - Lee, Seung Hoon

N1 - Funding Information: This research was supported by a grant (07142KFDA553) from Korea Food and Drug Administration in 2007 and partly by a Korea Basic Science Institute grant (KBSI-GJ-D28026 and K-MeP).

PY - 2009

Y1 - 2009

N2 - The amphetamine derivative 3, 4-methylenedioxymethamphetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblastoma cells after MDMA treatment. This elevated Atg5 expression interferes with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.

AB - The amphetamine derivative 3, 4-methylenedioxymethamphetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblastoma cells after MDMA treatment. This elevated Atg5 expression interferes with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.

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U2 - 10.1007/s10059-009-0075-2

DO - 10.1007/s10059-009-0075-2

M3 - Article

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AN - SCOPUS:66649106650

SN - 1016-8478

VL - 27

SP - 571

EP - 575

JO - Molecules and cells

JF - Molecules and cells

IS - 5

ER -

ATG5 expression induced by MDMA (ecstasy), interferes with neuronal differentiation of neuroblastoma cells

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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