Assessment of chromosomal losses and gains in hepatocellular carcinoma

Joon Chang; Nam Gyun Kim; Zhe Piao; Chanil Park; Kang Sik Park; Young Ki Paik; Woo Jung Lee; Byong Ro Kim; Hoguen Kim

doi:10.1016/S0304-3835(02)00083-6

Assessment of chromosomal losses and gains in hepatocellular carcinoma

Joon Chang, Nam Gyun Kim, Zhe Piao, Chanil Park, Kang Sik Park, Young Ki Paik, Woo Jung Lee, Byong Ro Kim, Hoguen Kim

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

We examined the chromosomal changes of 22 hepatocellular carcinomas (HCCs) by comparative genomic hybridization (CGH) analysis and compared the results with that of allelotype by polymerase chain reaction based loss of heterozygosity (PCR-LOH) analysis. By CGH analysis, frequent chromosomal losses were noted in the chromosomal region of 4q (59%), 8p (77%), and 16q (50%), whereas gains were noted in 1q (86%) and 8q (77%). All of these chromosomal arms were revealed to have frequent allelic imbalances by PCR-LOH analysis, however, 9% of chromosomal aberrations were detected only by CGH analysis and 2% were detected only by PCR-LOH analysis. Our results suggest that CGH analysis gives more precise results for the screening of chromosomal aberrations in HCCs than that of PCR-LOH analysis with randomly selected microsatellite markers.

Original language	English
Pages (from-to)	193-202
Number of pages	10
Journal	Cancer Letters
Volume	182
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3835(02)00083-6
Publication status	Published - 2002 Aug 28

Bibliographical note

Funding Information:
The authors are grateful to Mr. J. Lim for the statistical analysis and Miss J.E. Kim for technical assistance. This work was presented in part at the annual meeting of the American Association for Cancer Research, San Francisco, April 1–4, 2000 and supported by a grant from 21C Frontier Functional Analysis of Human Genome Project (FG1-4-01), Ministry of Science and Technology, South Korea.

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0304-3835(02)00083-6

Cite this

@article{51c2b01f9e514bb8be21d8162501166f,

title = "Assessment of chromosomal losses and gains in hepatocellular carcinoma",

abstract = "We examined the chromosomal changes of 22 hepatocellular carcinomas (HCCs) by comparative genomic hybridization (CGH) analysis and compared the results with that of allelotype by polymerase chain reaction based loss of heterozygosity (PCR-LOH) analysis. By CGH analysis, frequent chromosomal losses were noted in the chromosomal region of 4q (59%), 8p (77%), and 16q (50%), whereas gains were noted in 1q (86%) and 8q (77%). All of these chromosomal arms were revealed to have frequent allelic imbalances by PCR-LOH analysis, however, 9% of chromosomal aberrations were detected only by CGH analysis and 2% were detected only by PCR-LOH analysis. Our results suggest that CGH analysis gives more precise results for the screening of chromosomal aberrations in HCCs than that of PCR-LOH analysis with randomly selected microsatellite markers.",

author = "Joon Chang and Kim, {Nam Gyun} and Zhe Piao and Chanil Park and Park, {Kang Sik} and Paik, {Young Ki} and Lee, {Woo Jung} and Kim, {Byong Ro} and Hoguen Kim",

note = "Funding Information: The authors are grateful to Mr. J. Lim for the statistical analysis and Miss J.E. Kim for technical assistance. This work was presented in part at the annual meeting of the American Association for Cancer Research, San Francisco, April 1–4, 2000 and supported by a grant from 21C Frontier Functional Analysis of Human Genome Project (FG1-4-01), Ministry of Science and Technology, South Korea.",

year = "2002",

month = aug,

day = "28",

doi = "10.1016/S0304-3835(02)00083-6",

language = "English",

volume = "182",

pages = "193--202",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Assessment of chromosomal losses and gains in hepatocellular carcinoma

AU - Chang, Joon

AU - Kim, Nam Gyun

AU - Piao, Zhe

AU - Park, Chanil

AU - Park, Kang Sik

AU - Paik, Young Ki

AU - Lee, Woo Jung

AU - Kim, Byong Ro

AU - Kim, Hoguen

N1 - Funding Information: The authors are grateful to Mr. J. Lim for the statistical analysis and Miss J.E. Kim for technical assistance. This work was presented in part at the annual meeting of the American Association for Cancer Research, San Francisco, April 1–4, 2000 and supported by a grant from 21C Frontier Functional Analysis of Human Genome Project (FG1-4-01), Ministry of Science and Technology, South Korea.

PY - 2002/8/28

Y1 - 2002/8/28

N2 - We examined the chromosomal changes of 22 hepatocellular carcinomas (HCCs) by comparative genomic hybridization (CGH) analysis and compared the results with that of allelotype by polymerase chain reaction based loss of heterozygosity (PCR-LOH) analysis. By CGH analysis, frequent chromosomal losses were noted in the chromosomal region of 4q (59%), 8p (77%), and 16q (50%), whereas gains were noted in 1q (86%) and 8q (77%). All of these chromosomal arms were revealed to have frequent allelic imbalances by PCR-LOH analysis, however, 9% of chromosomal aberrations were detected only by CGH analysis and 2% were detected only by PCR-LOH analysis. Our results suggest that CGH analysis gives more precise results for the screening of chromosomal aberrations in HCCs than that of PCR-LOH analysis with randomly selected microsatellite markers.

AB - We examined the chromosomal changes of 22 hepatocellular carcinomas (HCCs) by comparative genomic hybridization (CGH) analysis and compared the results with that of allelotype by polymerase chain reaction based loss of heterozygosity (PCR-LOH) analysis. By CGH analysis, frequent chromosomal losses were noted in the chromosomal region of 4q (59%), 8p (77%), and 16q (50%), whereas gains were noted in 1q (86%) and 8q (77%). All of these chromosomal arms were revealed to have frequent allelic imbalances by PCR-LOH analysis, however, 9% of chromosomal aberrations were detected only by CGH analysis and 2% were detected only by PCR-LOH analysis. Our results suggest that CGH analysis gives more precise results for the screening of chromosomal aberrations in HCCs than that of PCR-LOH analysis with randomly selected microsatellite markers.

UR - http://www.scopus.com/inward/record.url?scp=0037190125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037190125&partnerID=8YFLogxK

U2 - 10.1016/S0304-3835(02)00083-6

DO - 10.1016/S0304-3835(02)00083-6

M3 - Article

C2 - 12048165

AN - SCOPUS:0037190125

SN - 0304-3835

VL - 182

SP - 193

EP - 202

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Assessment of chromosomal losses and gains in hepatocellular carcinoma

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this