Antinociceptive effects of transcytosed botulinum neurotoxin type a on trigeminal nociception in rats

Hye Jin Kim, Geun Woo Lee, Min Ji Kim, Kui Ye Yang, Seong Taek Kim, Yong Cheol Bae, Dong Kuk Ahn

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14 Citations (Scopus)


We examined the effects of peripherally or centrally administered botulinum neurotoxin type A (BoNT-A) on orofacial inflammatory pain to evaluate the antinociceptive effect of BoNT-A and its underlying mechanisms. The experiments were carried out on male Sprague-Dawley rats. Subcutaneous (3 U/kg) or intracisternal (0.3 or 1 U/kg) administration of BoNT-A significantly inhibited the formalin-induced nociceptive response in the second phase. Both subcutaneous (1 or 3 U/kg) and intracisternal (0.3 or 1 U/kg) injection of BoNT-A increased the latency of head withdrawal response in the complete Freund's adjuvant (CFA)-treated rats. Intracisternal administration of N-methyl-D-aspartate (NMDA) evoked nociceptive behavior via the activation of trigeminal neurons, which was attenuated by the subcutaneous or intracisternal injection of BoNT-A. Intracisternal injection of NMDA up-regulated c-Fos expression in the trigeminal neurons of the medullary dorsal horn. Subcutaneous (3 U/kg) or intracisternal (1 U/kg) administration of BoNT-A significantly reduced the number of c-Fos immunoreactive neurons in the NMDA-treated rats. These results suggest that the central antinociceptive effects the peripherally or centrally administered BoNT-A are mediated by transcytosed BoNT-A or direct inhibition of trigeminal neurons. Our data suggest that central targets of BoNT-A might provide a new therapeutic tool for the treatment of orofacial chronic pain conditions.

Original languageEnglish
Pages (from-to)349-355
Number of pages7
JournalKorean Journal of Physiology and Pharmacology
Issue number4
Publication statusPublished - 2015 Jul 1

Bibliographical note

Publisher Copyright:
Copyright © Korean J Physiol Pharmacol.

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology


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