Antiinflammatory activity of methanol extract isolated from stem bark of Magnolia kobus

Jong Soon Kane, Ki Hoon Lee, Mi Hwa Han, Hyunju Lee, Ji Mi Ahn, Sang Bae Han, Gyoonhee Han, Kiho Lee, Song Kyu Park, Hwan Mook Kim

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42 Citations (Scopus)


The present study reports the antiinflammatory activity of a methanol extract isolated from the stem bark of Magnolia kobus (MK). MK potently inhibited lipopolysaccharide (LPS)-induced production of nitric oxide and interleukin-1β (IL-1β) in RAW 264.7 cells, a murine macrophage-like cell line. The secretion of tumor necrosis factor-α (TNF-α) was also suppressed in LPS-stimulated RAW 264.7 cells although the magnitude of inhibition was weaker than that of nitric oxide and IL-1β. The mRNA expressions of inducible nitric oxide synthase (iNOS), IL-1β and TNF-α were also suppressed by MK in LPS-stimulated RAW 264.7 cells. Further study demonstrated that LPS-induced DNA binding of AP-1 and phosphorylation of c-jun TV-terminal kinase (JNK) were inhibited by MK treatment in RAW 264.7 cells, whereas phosphorylation of p38 mitogen-activated protein kinase was unaffected. Moreover, topical application of MK suppressed ear swelling in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation model. Collectively, these results suggest that MK exerts antiinflammatory effects in vitro and in vivo and this might be mediated, at least in part, by blocking AP-1 and JNK activation.

Original languageEnglish
Pages (from-to)883-888
Number of pages6
JournalPhytotherapy Research
Issue number7
Publication statusPublished - 2008 Jul

All Science Journal Classification (ASJC) codes

  • Pharmacology


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