Antibody-based surface plasmon resonance detection of intact viral pathogen

Hyoungwon Baac; József P. Hajós; Jennifer Lee; Donghyun Kim; Sung June Kim; Michael L. Shuler

doi:10.1002/bit.20882

Antibody-based surface plasmon resonance detection of intact viral pathogen

Hyoungwon Baac, József P. Hajós, Jennifer Lee, Donghyun Kim, Sung June Kim, Michael L. Shuler

Department of Electrical and Electronic Engineering

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus. Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two-channel (reference & test) micro-fluidic SPR system is used for reliable measurement. Bio-specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors.

Original language	English
Pages (from-to)	815-819
Number of pages	5
Journal	Biotechnology and Bioengineering
Volume	94
Issue number	4
DOIs	https://doi.org/10.1002/bit.20882
Publication status	Published - 2006 Jul 5

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1002/bit.20882

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title = "Antibody-based surface plasmon resonance detection of intact viral pathogen",

abstract = "Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus. Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two-channel (reference & test) micro-fluidic SPR system is used for reliable measurement. Bio-specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors.",

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AU - Baac, Hyoungwon

AU - Hajós, József P.

AU - Lee, Jennifer

AU - Kim, Donghyun

AU - Kim, Sung June

AU - Shuler, Michael L.

PY - 2006/7/5

Y1 - 2006/7/5

N2 - Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus. Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two-channel (reference & test) micro-fluidic SPR system is used for reliable measurement. Bio-specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors.

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Antibody-based surface plasmon resonance detection of intact viral pathogen

Abstract

All Science Journal Classification (ASJC) codes

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