Anti-cariogenic Characteristics of Rubusoside

Jeesoo Kim, Thi Thanh Hanh Nguyen, Juhui Jin, Iis Septiana, Gyu Min Son, Gang Hee Lee, You Jin Jung, Dilshad Qureshi, Il Kyoon Mok, Kunal Pal, Soo Yeon Yang, Seong Bo Kim, Doman Kim

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Streptococcus mutans plays an important role in the development of dental caries in humans by synthesizing adhesive insoluble glucans from sucrose by mutansucrase activity. To explore the anti-cariogenic characteristics of rubusoside (Ru), a natural sweetener component in Rubus suavissimus S. Lee (Rosaceae), we investigated the inhibitory effect of Ru against the activity of mutansucrase and the growth of Streptococcus mutans. Ru (50 mM) showed 97% inhibitory activity against 0.1 U/mL mutansucrase of S. mutans with 500 mM sucrose. It showed competitive inhibition with a Ki value of 1.1 ± 0.2 mM and IC50 of 2.3 mM. Its inhibition activity was due to hydrophobic and hydrogen bonding interactions based on molecular docking analysis. Ru inhibited the growth of S. mutans as a bacteriostatic agent, with MIC and MBC values of 6 mM and 8 mM, respectively. In addition, Ru showed synergistic anti-bacterial activity when it was combined with curcumin. Therefore, Ru is a natural anti-cariogenic agent with anti-mutansucrase activity and antimicrobial activity against S. mutans.

Original languageEnglish
Pages (from-to)282-287
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Issue number2
Publication statusPublished - 2019 Mar 1

Bibliographical note

Publisher Copyright:
© 2019, The Korean Society for Biotechnology and Bioengineering and Springer.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


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