ANGUSTIFOLIA mediates one of the multiple SCRAMBLED signaling pathways regulating cell growth pattern in Arabidopsis thaliana

Su Hwan Kwak, Sang Kee Song, Myeong Min Lee, John Schiefelbein

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5 Citations (Scopus)


In Arabidopsis thaliana, an atypical leucine-rich repeat receptor-like kinase, SCRAMBLED (SCM), is required for multiple developmental processes including root epidermal cell fate determination, silique dehiscence, inflorescence growth, ovule morphogenesis, and tissue morphology. Previous work suggested that SCM regulates these multiple pathways using distinct mechanisms via interactions with specific downstream factors. ANGUSTIFOLIA (AN) is known to regulate cell and tissue morphogenesis by influencing cortical microtubule arrangement, and recently, the AN protein was reported to interact with the SCM protein. Therefore, we examined whether AN might be responsible for mediating some of the SCM-dependent phenotypes. We discovered that both scm and an mutant lines cause an abnormal spiral or twisting growth of roots, but only the scm mutant affected root epidermal patterning. The siliques of the an and scm mutants also exhibited spiral growth, as previously reported, but only the scm mutant altered silique dehiscence. Interestingly, we discovered that the spiral growth of roots and siliques of the scm mutant is rescued by a truncated SCM protein that lacks its kinase domain, and that a juxtamembrane domain of SCM was sufficient for AN binding in the yeast two-hybrid analysis. These results suggest that the AN protein is one of the critical downstream factors of SCM pathways specifically responsible for mediating its effects on cell/tissue morphogenesis through cortical microtubule arrangement.

Original languageEnglish
Pages (from-to)587-593
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 2015 Sept 25

Bibliographical note

Funding Information:
This work was supported by the U.S. National Science Foundation (grant no. IOS-1121602 and IOS-1444400 to J.S. and S.H.K), and the faculty start-up fund from Long Island University, Brooklyn Campus (to S.H.K). We acknowledge the Arabidopsis Biological Resource Center (ABRC; Columbus, OH) for providing the SALK_086357 and SALK_026489 insertion line, and U15161 ANGUSTIFOLIA cDNA clone. We thank Gabriela Agama, Ramya Barre, Khushbu Patel, Ronisha Solanki, and Haley Tang for helpful assistance.

Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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