Analysis of cytomegalovirus-specific T-cell responses in patients with hypertension: Comparison of assay methods and antigens

Jong Chan Youn, Jun Yong Kim, Min Kyung Jung, Hee Tae Yu, Su Hyung Park, In Cheol Kim, Sun Ki Lee, Suk Won Choi, Seongwoo Han, Kyu Hyung Ryu, Sungha Park, Eui Cheol Shin

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7 Citations (Scopus)

Abstract

Background: Recent studies suggest an association between cytomegalovirus (CMV) infection and hypertension. In the present study, we used a variety of antigens and different assay methods to investigate the relationship between CMV-specific T-cell responses and arterial stiffness in patients with hypertension. Methods: To evaluate arterial stiffness, pulse wave velocity (PWV) was measured in 207 hypertensive patients (average age, 63 ± 8 years). To measure CMV pp65 and IE-1-specific T-cell responses, we performed intracellular cytokine staining (ICS) and enzyme-linked immunospot (ELISPOT) assays. We also analyzed CMV-specific T-cell responses against 10 different CMV antigens using ELISPOT assays. Results: In patients with hypertension, senescent CD8+ T-cell frequencies were significantly correlated with arterial stiffness. Moreover, arterial stiffness was independently associated with CMV pp65-specific CD8+ T-cell responses as measured by ICS. CMV-specific CD8+ T-cell responses measured by ICS and ELISPOT assays showed good agreement and significant correlation with each other. ELISPOT analyses against 10 different CMV antigens revealed a consistent response pattern irrespective of age, gender, and diabetes Conclusions: CMV pp65-specific CD8+ T-cell responses were independently correlated with arterial stiffness in patients with hypertension. Additionally, the results of ICS and ELISPOT assays showed a significant correlation and good agreement with each other. These findings are important for guiding choices regarding the broad clinical application of CMV-specific T-cell response assays in this patient population.

Original languageEnglish
Article number5
JournalClinical Hypertension
Volume24
Issue number1
DOIs
Publication statusPublished - 2018 Mar 20

Bibliographical note

Publisher Copyright:
© 2018 The Author(s).

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Cardiology and Cardiovascular Medicine

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