Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool widely used in monitoring cells, organelles, and tissues in biosciences. Since fluorescence lifetimes of most probes are a few nanoseconds, 20 ps measurement resolution is normally required. This requirement is quite challenging even with the fastest available optical and electronic devices, and several brilliant time-domain super-resolution techniques have been proposed for FLIM. The analog mean-delay (AMD) method is a recently introduced time-domain super-resolution technique for FLIM. Detailed constraints in the AMD method and their impact on the performance of the AMD super-resolution lifetime measurement are presented with experiments and simulations.
|Title of host publication
|Single Molecule Spectroscopy and Superresolution Imaging XII
|Felix Koberling, Zygmunt K. Gryczynski, Ingo Gregor
|Published - 2019
|Single Molecule Spectroscopy and Superresolution Imaging XII 2019 - San Francisco, United States
Duration: 2019 Feb 2 → 2019 Feb 3
|Progress in Biomedical Optics and Imaging - Proceedings of SPIE
|Single Molecule Spectroscopy and Superresolution Imaging XII 2019
|19/2/2 → 19/2/3
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging