An impedimetric biosensor for real-time monitoring of bacterial growth in a microbial fermentor

Young Hun Kim; Jae Sung Park; Hyo Il Jung

doi:10.1016/j.snb.2009.01.034

An impedimetric biosensor for real-time monitoring of bacterial growth in a microbial fermentor

Young Hun Kim, Jae Sung Park, Hyo Il Jung

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

In this paper, we describe an in situ sensor for estimating bacterial concentration in a lab-scale fermentor using impedance spectroscopy. We constructed the impedimetric biosensor using a gold-coated silicon wafer, PDMS polymer, and a borosilicate glass tube. An advantage of using these materials was that we could make a plug-type, disposable electrode. We compared the impedimetric differences between bacterial cells, culture media, and metabolic by-products. We measured real-time impedance changes of an E. coli solution at 0.01 MHz frequency of applied AC for 13 h, and correlated them with the bacteria concentration as estimated by a hemocytometer. In conclusion, impedance increased and reactance decreased during the measurement period, as the concentration of E. coli increased. The correlation between the E. coli concentration and the impedimetric data was linear.

Original language	English
Pages (from-to)	270-277
Number of pages	8
Journal	Sensors and Actuators, B: Chemical
Volume	138
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2009.01.034
Publication status	Published - 2009 Apr 24

Bibliographical note

Funding Information:
This work was supported by National Core Research Center (NCRC) for Nanomedical Technology of the Korea Science & Engineering Foundation (Grant no. R15-2004-024-01001-0) and Seoul Research & Business Development (Seoul R&BD Program, 11128).

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2009.01.034

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title = "An impedimetric biosensor for real-time monitoring of bacterial growth in a microbial fermentor",

abstract = "In this paper, we describe an in situ sensor for estimating bacterial concentration in a lab-scale fermentor using impedance spectroscopy. We constructed the impedimetric biosensor using a gold-coated silicon wafer, PDMS polymer, and a borosilicate glass tube. An advantage of using these materials was that we could make a plug-type, disposable electrode. We compared the impedimetric differences between bacterial cells, culture media, and metabolic by-products. We measured real-time impedance changes of an E. coli solution at 0.01 MHz frequency of applied AC for 13 h, and correlated them with the bacteria concentration as estimated by a hemocytometer. In conclusion, impedance increased and reactance decreased during the measurement period, as the concentration of E. coli increased. The correlation between the E. coli concentration and the impedimetric data was linear.",

keywords = "Bacterial growth, Disposable biosensor, Fermentation, Impedimetric biosensor, Real-time monitoring",

author = "Kim, {Young Hun} and Park, {Jae Sung} and Jung, {Hyo Il}",

note = "Funding Information: This work was supported by National Core Research Center (NCRC) for Nanomedical Technology of the Korea Science & Engineering Foundation (Grant no. R15-2004-024-01001-0) and Seoul Research & Business Development (Seoul R&BD Program, 11128). ",

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AU - Kim, Young Hun

AU - Park, Jae Sung

AU - Jung, Hyo Il

N1 - Funding Information: This work was supported by National Core Research Center (NCRC) for Nanomedical Technology of the Korea Science & Engineering Foundation (Grant no. R15-2004-024-01001-0) and Seoul Research & Business Development (Seoul R&BD Program, 11128).

PY - 2009/4/24

Y1 - 2009/4/24

N2 - In this paper, we describe an in situ sensor for estimating bacterial concentration in a lab-scale fermentor using impedance spectroscopy. We constructed the impedimetric biosensor using a gold-coated silicon wafer, PDMS polymer, and a borosilicate glass tube. An advantage of using these materials was that we could make a plug-type, disposable electrode. We compared the impedimetric differences between bacterial cells, culture media, and metabolic by-products. We measured real-time impedance changes of an E. coli solution at 0.01 MHz frequency of applied AC for 13 h, and correlated them with the bacteria concentration as estimated by a hemocytometer. In conclusion, impedance increased and reactance decreased during the measurement period, as the concentration of E. coli increased. The correlation between the E. coli concentration and the impedimetric data was linear.

AB - In this paper, we describe an in situ sensor for estimating bacterial concentration in a lab-scale fermentor using impedance spectroscopy. We constructed the impedimetric biosensor using a gold-coated silicon wafer, PDMS polymer, and a borosilicate glass tube. An advantage of using these materials was that we could make a plug-type, disposable electrode. We compared the impedimetric differences between bacterial cells, culture media, and metabolic by-products. We measured real-time impedance changes of an E. coli solution at 0.01 MHz frequency of applied AC for 13 h, and correlated them with the bacteria concentration as estimated by a hemocytometer. In conclusion, impedance increased and reactance decreased during the measurement period, as the concentration of E. coli increased. The correlation between the E. coli concentration and the impedimetric data was linear.

KW - Bacterial growth

KW - Disposable biosensor

KW - Fermentation

KW - Impedimetric biosensor

KW - Real-time monitoring

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An impedimetric biosensor for real-time monitoring of bacterial growth in a microbial fermentor

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Bibliographical note

All Science Journal Classification (ASJC) codes

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