Abstract
In this report, we have studied the effectiveness of field-target overlap to evaluate detection sensitivity of surface plasmon resonance (SPR) biosensors. The investigation used theoretical analysis based on the transfer matrix method, which was experimentally confirmed by thin film-based detection in sandwich and reverse sandwich immunoglobulin G (IgG) assays. Both theoretical and experimental results show that strong correlation exists between the overlap and the sensitivity with the coefficient of correlation higher than 95% in all the cases that we have considered. We have also confirmed the correlation in diffraction grating-based SPR measurement of IgG/anti-IgG interactions. The correlation elucidates the mechanism behind the far-field detection sensitivity of SPR biosensors and can lead to the enhancement of SPR biosensing with molecular scale sensitivity.
| Original language | English |
|---|---|
| Pages (from-to) | 4626-4631 |
| Number of pages | 6 |
| Journal | Optics Communications |
| Volume | 285 |
| Issue number | 21-22 |
| DOIs | |
| Publication status | Published - 2012 Oct 1 |
Bibliographical note
Funding Information:This work was supported by the National Research Foundation (NRF) grants funded by the Korean Government ( 2009-0070732, 2010-0007993, and 2011-0017500 ).
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Physical and Theoretical Chemistry
- Electrical and Electronic Engineering